Gel Filtration Desalting/Buffer Exchange Columns and Bed sizes
PhyTip gel filtration columns are used to treat proteins and other large molecules to remove the solution buffer or exchange the solution buffer with a new desired buffer in existing 96-well systems, or the 12-well PhyNexus MEA liquid handler. The buffer can be removed or exchanged from a sample without harming (denaturing or changing the structure) of the protein, protein fragment, etc. Small sample volumes are preserved without dilution. The PhyTip technology is compatible with all existing major robotic liquid handlers. Methods may be automated with other purification steps in an integrated workflow. For example, the technology may be integrated with other PhyNexus automated processes such as tagging or purification. The gel filtration columns are available in 5 Kd or 10 Kd resins.
|Bed Size||Sample Size|
|200 μL||20-90 μL|
|600 μL||100-400 μL|