1
2
3
1

Top Frit

2

Resin bed

3

Bottom Frit

Gel filtration Desalting/Buffer Exchange Columns and Bed sizes

PhyTip gel filtration columns are used to treat proteins and other large molecules to remove the solution buffer or exchange the solution buffer with a new desired buffer in existing 96-well systems, or the 12-well PhyNexus MEA liquid handler. The buffer can be removed or exchanged from a sample without harming (denaturing or changing the structure) of the protein, protein fragment, etc. Small sample volumes are preserved without dilution. The PhyTip technology is compatible with all existing major robotic liquid handlers. Methods may be automated with other purification steps in an integrated work-for. For example, the technology may be integrated with other PhyNexus automated processes such as tagging or purification. The columns are available in 5 Kd or 10 Kd resins.

Bed SizeSample Size
200 μL20-90 μL
600 μL100-400 μL