Use of a Protein Engineering Strategy to Overcome Limitations in the Production of ‘Difficult to Express’ Recombinant Proteins.

Biotechnology and Bioengineering, July 2017

Auto-induction of Pichia pastoris AOX1 promoter for membrane protein expression

Protein Expression and Purification, June 2017

Streamlining workflow and automation to accelerate laboratory scale protein production.

Protein Expression and Purification, Vol 133, 160-169, May 2017.

The Tetrameric Plant Lectin BanLec Neutralizes HIV through Bidentate Binding to Specific Viral Glycans.

Structure (London, England : 1993), Vol 46, 233-239, Apr 2017.

Modulation and modeling of monoclonal antibody N-linked glycosylation in mammalian cell perfusion reactors.

Biotechnology and Bioengineering, Apr 2017.

Parallel experimental design and multivariate analysis provides efficient screening of cell culture media supplements to improve Biosimilar product quality.

Biotechnology and Bioengineering, Feb 2017.

Characterization of H type 1 and type 1 N-acetyllactosamine glycan epitopes on ovarian cancer specifically recognized by the anti-glycan monoclonal antibody mAb-A4.

The Journal of Biological Chemistry, Feb 2017.

Capillary electrophoresis analysis of N-glycosylation changes of serum paraproteins in multiple myeloma.

Electrophoresis, Jan 2017.

Excess reactive oxygen species production mediates monoclonal antibody-induced human embryonic stem cell death via oncosis.

Cell Death and Differentiation, Jan 2017.

Discovery of a potent BTK inhibitor with a novel binding mode using parallel selections with a DNA-encoded chemical library.

ChemBioChem, Jan 2017.

Quantitative Glycomics: A Combined Analytical and Bioinformatics Approach.

Methods in Enzymology, 2017.

CP40 from Corynebacterium pseudotuberculosis is an endo-$\beta$-N-acetylglucosaminidase.

BMC Microbiology, Vol 16, 261, Dec 2016.

Twoplex 12/13 C 6 aniline stable isotope and linkage specific sialic acid labelling 2D-LC-MS workflow for quantitative N –glycomics.

Proteomics, Nov 2016.

Discovery of cofactor-specific, bactericidal Mycobacterium tuberculosis InhA inhibitors using DNA-encoded library technology.

Proceedings of the National Academy of Sciences of the United States of America, Nov 2016.

Robust factor selection in early cell culture process development for the production of a biosimilar monoclonal antibody.

Biotechnology Progress, Sep 2016.

Process performance and product quality in an integrated continuous antibody production process.

Biotechnology and Bioengineering, Aug 2016.

Identifying a Robust Design Space for Glycosylation During Monoclonal Antibody Production.

Biotechnology Progress, Jun 2016.

EndoSd: an IgG glycan hydrolyzing enzyme in Streptococcus dysgalactiae subspecies dysgalactiae.

Future Microbiology, May 2016.

High-throughput analysis of immunoglobulin G glycosylation.

Expert Review of Proteomics, Vol 13, No 5, 523-534, May 2016.

Downstream process development strategies for effective bioprocesses: Trends, progress, and combinatorial approaches.

Engineering in Life Sciences, Apr 2016.

Rapid N-glycan release from glycoproteins using immobilized PNGase F microcolumns.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, Feb 2016.

Implementation of an Automated High-Throughput Plasmid DNA Production Pipeline.

Journal of Laboratory Automation, Feb 2016.

Rapid N-glycan release from glycoproteins using immobilized PNGase F microcolumns.

Journal of Chromatography B, Feb 2016.

From haystack to needle: finding value with DNA encoded library technology at GSK.

Med. Chem. Commun., Vol 11, 296-307, 2016.

Analysis of the Productivity of DNA Encoded Libraries.

Med. Chem. Commun., 2016.

Robust factor selection in early cell culture process development for the production of a biosimilar monoclonal antibody.

Biotechnology Progress, 2016.

Expression, purification, and characterization of recombinant human and murine milk fat globule-epidermal growth factor-factor 8.

Protein Expression and Purification, Vol 124, 10-22, 2016.

Automated screening for small organic ligands using DNA-encoded chemical libraries.

Nature Protocols, Vol 11, No 4, 764-780, 2016.

The Type IV Pilus Assembly ATPase PilB of Myxococcus xanthus interacts with the Inner Membrane Platform Protein PilC and the Nucleotide Binding Protein PilM.

Journal of Biological Chemistry, No 6, 25, 2016.

Evaluating high-throughput scale-down chromatography platforms for increased process understanding.

Engineering in Life Sciences, 2016.

A Combination of Structural and Empirical Analyses Delineates the Key Contacts Mediating Stability and Affinity Increases in an Optimized Biotherapeutic Single-chain Fv (scFv).

Journal of Biological Chemistry, Vol 291, No 3, 1267-1276, 2016.

Lessons learned in building high throughput process development capabilities.

Engineering in Life Sciences, 2016.

Current advances in the development of high-throughput purification strategies for the generation of therapeutic antibodies.

Pharmaceutical Bioprocessing, Vol 3, No 6, 411-424, Oct 2015.

DNA Encoded Library Selections and Insights Provided by Computational Simulations.

ACS Chemical Biology, Jul 2015.

High-throughput purification tools for rapid upstream process development are interchangeable for biologics.

Engineering in Life Sciences, Jul 2015.

Improved assembly of bispecific antibodies from knob and hole half-antibodies.

Biotechnology Progress, Jul 2015.

Comparative glycoprofiling of HIV gp120 immunogens by capillary electrophoresis and MALDI mass spectrometry.

Electrophoresis, Vol 36, No 11-12, 1305-1313, Jun 2015.

Encoded library technology screening of hepatitis C virus NS4B yields a small-molecule compound series with in vitro replicon activity.

Antimicrobial agents and chemotherapy, Vol 59, No 6, 3450-3459, Jun 2015.

Poly(N-vinylimidazole/ethylene glycol dimethacrylate) for the purification and isolation of phenolic acids.

Analytica Chimica Acta, Vol 885, 199-206, Jun 2015.

Use of the site-specific retargeting jump-in platform cell line to support biologic drug discovery.

Journal of biomolecular screening, Vol 20, No 4, 528-535, Apr 2015.

A simplified and robust protocol for immunoglobulin expression in Escherichia coli cell-free protein synthesis systems.

Biotechnology Progress, Vol 31, No 3, 823-831, 2015.

Sample Preparation for N-Glycosylation Analysis of Therapeutic Monoclonal Antibodies by Electrophoresis.

Methods in Molecular Biology, Vol 1274, 183-195, 2015.

Generation of potent mouse monoclonal antibodies to self-proteins using T-cell epitope “tags.”

mAbs, Vol 7, No 1, 129-137, 2015.

Augmented Binary Substitution: Single-pass CDR germ-lining and stabilization of therapeutic antibodies.

Proceedings of the National Academy of Sciences of the United States of America, Vol 112, No 50, 15354-15359, 2015.

Optimisation of a simple method to transiently transfect a CHO cell line in high-throughput and at large scale.

Protein Expression and Purification, 2015.

Structure of a highly acidic $\beta$-lactamase from the moderate halophile Chromohalobacter sp. 560 and the discovery of a Cs  -selective binding site,

Acta Crystallographica Section D: Biological Crystallography, Vol 71, No 3, 541-554, 2015.

Automated pipeline for rapid production \& screening of HIV-specific monoclonal antibodies using Pichia pastoris.

Biotechnology and Bioengineering, 2015.

Identification of anti-tumour biologics using primary tumour models, 3-D phenotypic screening and image-based multi-parametric profiling.

Molecular Cancer, Vol 14, No 1, 147, 2015.

Arginine methylation and citrullination of splicing factor proline- and glutamine-rich ( SFPQ / PSF ) regulates its association with mRNA.

RNA, Vol 21, No 3, 1-13, 2015.

Generation of novel monoclonal antibodies for the enrichment and characterization of human corneal endothelial cells (hCENC) necessary for the treatment of corneal endothelial blindness.

mAbs, Vol 6, No 6, 1439-1452, Nov 2014.

Production of Site-Specific Antibody–Drug Conjugates Using Optimized Non-Natural Amino Acids in a Cell-Free Expression System.

Bioconjugate Chemistry, Vol 25, No 2, 351-361, Feb 2014.

Recent advances in the analysis of therapeutic proteins by capillary and microchip electrophoresis.

Anal Methods., Vol 6, No 15, 5427-49, 2014.

Phage display and hybridoma generation of antibodies to human CXCR2 yields antibodies with distinct mechanisms and epitopes.

mAbs, Vol 6, No 6, 1425-1438, 2014.

Encoded library technology as a source of hits for the discovery and lead optimization of a potent and selective class of bactericidal direct inhibitors of mycobacterium tuberculosis inha.

Journal of Medicinal Chemistry, Vol 57, No 4, 1276-1288, 2014.
Remediating agitation-induced antibody aggregation by eradicating exposed hydrophobic motifs.

mAbs, Vol 6, No 6, 1540-1550, 2014.

Galactosyltransferase 4 is a major control point for glycan branching in N-linked glycosylation.

Journal of Cell Science, Vol 127, No 23, 5014-5026, 2014.

Strategy for selecting disposable bags for cell culture media applications based on a root-cause investigation.

Biotechnology Progress, Vol 29, No 6, 1535-1549, Nov 2013.

The impact of microcarrier culture optimization on the glycosylation profile of a monoclonal antibody.

SpringerPlus, Vol 2, No 1, 25, 2013.

Combining phenotypic and proteomic approaches to identify membrane targets in a ‘triple negative’ breast cancer cell type.

Molecular cancer, Vol 12, No 1, 11, 2013.

CDR-restricted engineering of native human scFvs creates highly stable and soluble bifunctional antibodies for subcutaneous delivery.

mAbs, Vol 5, No 6, 882-895, 2013.

Automated sample preparation for CE-SDS.

Electrophoresis, Vol 34, No 9-10, 1369-1374, 2013.

Facile fabrication and instant application of miniaturized antibody-decorated affinity columns for higher-order structure and functional characterization of TRIM21 epitope peptides.

Analytical Chemistry, Vol 85, No 21, 10479-10487, 2013.

High-throughput process development of chromatography steps: Advantages and limitations of different formats used.

Biotechnology Journal, Vol 7, No 10, 1192-1202, Oct 2012.

Method for Recovery and Immunoaffinity Enrichment of Membrane Proteins Illustrated with Metastatic Ovarian Cancer Tissues.

International Journal of Proteomics, Vol 2012, 1-15, 2012.

Chip-based CE for rapid separation of 8-aminopyrene-1,3,6-trisulfonic acid (APTS) derivatized glycans.

Electrophoresis, Vol 31, No 22, 3783-3786, Nov 2010.

Mass Spectrometric-Based Stable Isotopic 2-Aminobenzoic Acid Glycan Mapping for Rapid Glycan Screening of Biotherapeutics.

Analytical Chemistry, Vol 82, No 4, 1498-1508, Feb 2010.

Widening the bottleneck: Increasing success in protein expression and purification.

Journal of Structural Biology, Vol 172, No 1, 14-20, 2010.

RNA Purification and Analysis.

Gjerde et al., Wiley-VCH, Jul 2009.

Ion Chromatography.

Fritz et al., Wiley-VCH, Feb 2009.

Fluorescent isotope-coded affinity tag 2: Peptide labeling and affinity capture.

Electrophoresis, Vol 30, No 7, 1111-1118, 2009.

Enrichment of Amadori products derived from the nonenzymatic glycation of proteins using microscale boronate affinity chromatography.

Vol 393, No 1, 8-22, 2009.

High-throughput immunoglobulin G N-glycan characterization using rapid resolution reverse-phase chromatography tandem mass spectrometry.

Analytical Biochemistry, Vol 385, No 1, 69-79, 2009.

Fluorescent isotope-coded affinity tag (FCAT) I: Design and synthesis.

Bioorganic Chemistry, Vol 36, No 6, 299-311, Dec 2008.

A simple high-throughput purification method for hit identification in protein screening.

Journal of Immunological Methods, Vol 339, No 1, 38-46, Nov 2008.

Selection Against Undifferentiated Human Embryonic Stem Cells by a Cytotoxic Antibody Recognizing Podocalyxin-Like Protein-1.

Stem Cells, Vol 26, No 6, 1454-1463, Jun 2008.

Boronic acid lectin affinity chromatography (BLAC). 2. Affinity micropartitioning-mediated comparative glycosylation profiling.

Analytical and Bioanalytical Chemistry, Vol 392, No 1-2, 195-201, 2008.

Sample preparation for the analysis of complex carbohydrates by multicapillary gel electrophoresis with light-emitting diode induced fluorescence detection.

Analytical Chemistry, Vol 80, No 11, 4241-4246, 2008.

A microscale yeast cell disruption technique for integrated process development strategies.

Biotechnology Progress, Vol 24, No 3, 606-614, 2008.

Boronic acid-lectin affinity chromatography. 1. Simultaneous glycoprotein binding with selective or combined elution.

Analytical and Bioanalytical Chemistry, Vol 389, No 7-8, 2097-2102, 2007.

Automated sample preparation facilitated by PhyNexus MEA purification system for oligosaccharide mapping of glycoproteins.

Analytical Biochemistry, Vol 369, No 2, 202-209, 2007.

An automated microscale chromatographic purification of virus-like particles as a strategy for process development.

Biotechnology and applied biochemistry, Vol 47, No Pt2, 131-139, 2007.

Micro-scale open-tube capillary separations of functional proteins.

Analytical Biochemistry, Vol 350, No 1, 128-137, 2006.

High-throughput affinity ranking of antibodies using surface plasmon resonance microarrays.

Analytical Biochemistry, Vol 351, No 2, 241-253, 2006.

Material-Enhanced Laser Desorption/Ionization (MELDI)-A New Protein Profiling Tool Utilizing Specific Carrier Materials for Time of Flight Mass Spectrometric Analysis.

Journal of the American Society for Mass Spectrometry, Vol 17, No 9, 1203-1208, 2006.

First Meeting on Developments in Protein Interaction Analysis (DiPIA 2005).

Expert Review of Proteomics, Vol 2, No 6, 833-835, Dec 2005.

Striving for purity: advances in protein purification.

Nature Methods, Vol 2, No 1, 71-77, 2005.

Drug discovery – antibody enrichment and characterization

Genetic Engineering News, Vol 25, No 1, 2005.

Protein purification: pure but not simple.

Nature, Vol 434, No 7034, 795-798, 2005.