Automated Expression Screening: From Plasmid DNA to Protein Purification with PhyTip columns.

Lee Hoang, Ph.D. | Director of R&D, Biomolecules

PhyNexus technology for small-scale purification of proteins and antibodies is particularly amenable to expression screening. Fully automated PhyTip columns are used for purification of 96 plasmid DNA constructs, which are suitable for small scale transient transfection of mammalian and insect cells or transformation of yeast and E. coli. The proteins from these expressions can then be purified by automated PhyTip columns to complete the screen, because expression is only valuable if the protein is purifiable.