GST-tagged Proteins - Glutathione Columns - PhyNexus Automation | For Faster Research

Glutathione Columns

Glutathione PhyTip Columns demonstrate high binding affinity and high capacity for the affinity purification of functional recombinant GST-tagged proteins.

Glutathione columns routinely produce samples of greater than 90% purity.
60-80% recovery of sample proteins.
Glutathione PhyTip columns achieve high levels of purity even in the presence of 40X excess BSA
High yields are obtained regardless of the level of contaminant proteins in the sample
Note that the proper use of Glutathione PhyTip columns requires slow flow rates of 0.06mL/min

Request A Quote

Description

Glutathione PhyTip columns have been optimized for use with specific PhyNexus reagents and instrument flow rates/volumes as shown below. This information was collected using the PhyTip ME 1000 and ME 200 Purification Systems.

NOTE THAT FLOW RATE IS OF CRITICAL IMPORTANCE WHEN PERFORMING GLUTATHIONE-BASED SEPARATIONS; SLOWER FLOW RATES ARE TYPICALLY REQUIRED AS COMPARED TO OTHER RESINS!

200+ PhyTip columns with Protein A resin:

A 200 µL E. coli lysate sample with 6 µg GST was processed using the conditions shown below. Greater than 20% of the original GST mass is recovered in the final sample volume. In addition, this recovered GST is purified to >95% purity as determined by HPLC with UV absorbance detection at 215 nm.

Capture: 200 µL sample is processed by passing through the resin bed for two cycles at 60 uL/minute.

Purify: 200 µL of PhyNexus Glutathione Wash Buffer I is passed through the resin bed for two cycles at 500 µL/min, followed by a second wash with the same buffer passed through the resin bed for two cycles at 500 µL/min.

Enrich: elute the protein into solution with 10µL of PhyNexus Glutathione Elution Buffer passed through the resin bed for four cycles at a flow rate of 500 µL/min.

1000+ PhyTip columns with Protein A resin:

A 500 µL E. coli lysate sample with 15 µg GST was processed using the conditions shown below. Greater than 20% of the original GST mass is recovered in the final sample volume. In addition, this recovered GST is purified to >95% purity as determined by HPLC with UV absorbance detection at 215 nm.

Capture: 500 µL sample is processed by passing through the resin bed for two cycles at 60 uL/minute.

Purify: 1000 µL of PhyNexus Glutathione Wash Buffer I is passed through the resin bed for two cycles at 500 µL/min, followed by a second wash with the same buffer passed through the resin bed for two cycles at 500 µL/min.

Enrich: elute the protein into solution with 15µL of PhyNexus Glutathione Elution Buffer passed through the resin bed for four cycles at a flow rate of 500 µL/min.

Data

Shipping and Storage

Each pack of PhyTip columns has been manufactured and QC’d to the highest standards and shipped in retainer boxes that maintain the integrity of the specific affinity resin within each PhyTip column. This product is shipped at ambient temperatures, but on receipt should be stored in a standard laboratory refrigerator between 4 and 8◦C.

Do NOT freeze or store frozen.
When not in use, keep the lid of the box closed and sealed, store in the refrigerator.
Do not allow affinity resin to dry out by extended storage in a dry environment.

Glutathione PhyTip columns are stored in Glycerol when shipped from PhyNexus.

Documents

Glutathione Product Insert Sheet

Glutathione Flyer

Glutathione Spec Sheet

Glutathione PhyTip Columns MSDS

Robotics Systems Compatibility

GST-tagged Protein PhyTip Columns are available for these robotic systems.

Robotic Systems
Agilent Velocity 11 Bravo & V-Prep
Beckman FX & NX 96-well
Caliper SciClone & Zephyr 96-channel (now Perkin Elmer)
Dynamic Devices Oasis & Lynx
Hamilton STAR & Nimbus
Perkin Elmer Janus Varispan 4 or 8-well
Perkin Elmer Packard MiniTrack & Janus MDT 96-channel
PhyNexus MEA or ME
Tecan Freedom Evo LiHa & Tecan Te-MO
Tecan Freedom Evo MCA 96-well

Part Numbers

Glutathione PhyTip Columns

Resin	Robotic System	Tip Volume	QTY/Box	Resin Volume	Part Number	Description
Glutathione	Agilent Velocity 11 Bravo & V-Prep	200	96	20	PTV 92-20-04	Box of 96 PhyTip columns (200 µL volume) 20 µL of Glutathione affinity resin per column
Glutathione	Beckman FX & NX 96-well	200	96	5	PTB 92-05-04	Box of 96 PhyTip columns (200 µL volume) 5 µL of Glutathione affinity resin per column
Glutathione	Beckman FX & NX 96-well	200	96	20	PTB 92-20-04	Box of 96 PhyTip columns (200 µL volume) 20 µL of Glutathione affinity resin per column
Glutathione	Caliper SciClone & Zephyr 96 channel_now Perkin Elmer	200	96	5	PTZ 92-05-04	Box of 96 PhyTip columns (200 µL volume) 5 µL of Glutathione affinity resin per column
Glutathione	Caliper SciClone & Zephyr 96 channel_now Perkin Elmer	200	96	20	PTZ 92-20-04	Box of 96 PhyTip columns (200 µL volume) 20 µL of Glutathione affinity resin per column
Glutathione	Dynamic Devices Oasis & Lynx	1000	96	10	PTD 91-10-04	Box of 96 PhyTip columns (1 mL volume) 10 µL of Glutathione affinity resin per column
Glutathione	Dynamic Devices Oasis & Lynx	1000	96	20	PTD 91-20-04	Box of 96 PhyTip columns (1 mL volume) 20 µL of Glutathione affinity resin per column
Glutathione	Dynamic Devices Oasis & Lynx	1000	96	40	PTD 91-40-04	Box of 96 PhyTip columns (1 mL volume) 40 µL of Glutathione affinity resin per column
Glutathione	Dynamic Devices Oasis & Lynx	1000	96	80	PTD 91-80-04	Box of 96 PhyTip columns (1 mL volume) 80 µL of Glutathione affinity resin per column
Glutathione	Dynamic Devices Oasis & Lynx	1000	96	160	PTD 91-16-04	Box of 96 PhyTip columns (1 mL volume) 160 µL of Glutathione affinity resin per column
Glutathione	Dynamic Devices Oasis & Lynx	200	96	5	PTD 92-05-04	Box of 96 PhyTip columns (200 µL volume) 5 µL of Glutathione affinity resin per column
Glutathione	Dynamic Devices Oasis & Lynx	200	96	20	PTD 92-20-04	Box of 96 PhyTip columns (200 µL volume) 20 µL of Glutathione affinity resin per column
Glutathione	Hamilton STAR & Nimbus	1000	96	10	PTH 91-10-04	Box of 96 PhyTip columns (1 mL volume) 10 µL of Glutathione affinity resin per column
Glutathione	Hamilton STAR & Nimbus	1000	96	20	PTH 91-20-04	Box of 96 PhyTip columns (1 mL volume) 20 µL of Glutathione affinity resin per column
Glutathione	Hamilton STAR & Nimbus	1000	96	40	PTH 91-40-04	Box of 96 PhyTip columns (1 mL volume) 40 µL of Glutathione affinity resin per column
Glutathione	Hamilton STAR & Nimbus	1000	96	80	PTH 91-80-04	Box of 96 PhyTip columns (1 mL volume) 80 µL of Glutathione affinity resin per column
Glutathione	Hamilton STAR & Nimbus	1000	96	160	PTH 91-16-04	Box of 96 PhyTip columns (1 mL volume) 160 µL of Glutathione affinity resin per column
Glutathione	Hamilton STAR & Nimbus	300	96	5	PTH 93-05-04	Box of 96 PhyTip columns (300 µL volume) 5 µL of Glutathione affinity resin per column
Glutathione	Hamilton STAR & Nimbus	300	96	20	PTH 93-20-04	Box of 96 PhyTip columns (300 µL volume) 20 µL of Glutathione affinity resin per column
Glutathione	Perkin Elmer Janus Varispan 4 or 8-well	1000	96	10	PTJ 91-10-04	Box of 96 PhyTip columns (1 mL volume) 10 µL of Glutathione affinity resin per column
Glutathione	Perkin Elmer Janus Varispan 4 or 8-well	1000	96	20	PTJ 91-20-04	Box of 96 PhyTip columns (1 mL volume) 20 µL of Glutathione affinity resin per column
Glutathione	Perkin Elmer Janus Varispan 4 or 8-well	1000	96	40	PTJ 91-40-04	Box of 96 PhyTip columns (1 mL volume) 40 µL of Glutathione affinity resin per column
Glutathione	Perkin Elmer Janus Varispan 4 or 8-well	1000	96	80	PTJ 91-80-04	Box of 96 PhyTip columns (1 mL volume) 80 µL of Glutathione affinity resin per column
Glutathione	Perkin Elmer Janus Varispan 4 or 8-well	1000	96	160	PTJ 91-16-04	Box of 96 PhyTip columns (1 mL volume) 160 µL of Glutathione affinity resin per column
Glutathione	Perkin Elmer Packard MiniTrack & Janus MDT 96 channel	200	96	5	PTP 92-05-04	Box of 96 PhyTip columns (200 µL volume) 5 µL of Glutathione affinity resin per column
Glutathione	Perkin Elmer Packard MiniTrack & Janus MDT 96 channel	200	96	20	PTP 92-20-04	Box of 96 PhyTip columns (200 µL volume) 20 µL of Glutathione affinity resin per column
Glutathione	PhyNexus MEA or ME	1000	96	10	PTR 91-10-04	Box of 96 PhyTip columns (1 mL volume) 10 µL of Glutathione affinity resin per column
Glutathione	PhyNexus MEA or ME	1000	96	20	PTR 91-20-04	Box of 96 PhyTip columns (1 mL volume) 20 µL of Glutathione affinity resin per column
Glutathione	PhyNexus MEA or ME	1000	96	40	PTR 91-40-04	Box of 96 PhyTip columns (1 mL volume) 40 µL of Glutathione affinity resin per column
Glutathione	PhyNexus MEA or ME	1000	96	80	PTR 91-80-04	Box of 96 PhyTip columns (1 mL volume) 80 µL of Glutathione affinity resin per column
Glutathione	PhyNexus MEA or ME	1000	96	160	PTR 91-16-04	Box of 96 PhyTip columns (1 mL volume) 160 µL of Glutathione affinity resin per column
Glutathione	PhyNexus MEA or ME	1000	96	320	PTR 91-32-04	Box of 96 PhyTip columns (1 mL volume) 320 µL of Glutathione affinity resin per column
Glutathione	PhyNexus MEA or ME	200	96	5	PTR 92-05-04	Box of 96 PhyTip columns (200 µL volume) 5 µL of Glutathione affinity resin per column
Glutathione	PhyNexus MEA or ME	200	96	20	PTR 92-20-04	Box of 96 PhyTip columns (200 µL volume) 20 µL of Glutathione affinity resin per column
Glutathione	Tecan Freedom Evo LiHa & Tecan Te-MO	1000	96	10	PTT 91-10-04	Box of 96 PhyTip columns (1 mL volume) 10 µL of Glutathione affinity resin per column
Glutathione	Tecan Freedom Evo LiHa & Tecan Te-MO	1000	96	20	PTT 91-20-04	Box of 96 PhyTip columns (1 mL volume) 20 µL of Glutathione affinity resin per column
Glutathione	Tecan Freedom Evo LiHa & Tecan Te-MO	1000	96	40	PTT 91-40-04	Box of 96 PhyTip columns (1 mL volume) 40 µL of Glutathione affinity resin per column
Glutathione	Tecan Freedom Evo LiHa & Tecan Te-MO	1000	96	80	PTT 91-80-04	Box of 96 PhyTip columns (1 mL volume) 80 µL of Glutathione affinity resin per column
Glutathione	Tecan Freedom Evo LiHa & Tecan Te-MO	1000	96	160	PTT 91-16-04	Box of 96 PhyTip columns (1 mL volume) 160 µL of Glutathione affinity resin per column
Glutathione	Tecan Freedom Evo LiHa & Tecan Te-MO	200	96	5	PTT 92-05-04	Box of 96 PhyTip columns (200 µL volume) containing 5 µL of Glutathione affinity resin per column
Glutathione	Tecan Freedom Evo LiHa & Tecan Te-MO	200	96	20	PTT 92-20-04	Box of 96 PhyTip columns (200 µL volume) containing 20 µL of Glutathione affinity resin per column
Glutathione	Tecan Freedom Evo MCA 96-well	200	96	5	PTM 92-05-04	Box of 96 PhyTip columns (200 µL volume) 5 µL of Glutathione affinity resin per column
Glutathione	Tecan Freedom Evo MCA 96-well	200	96	20	PTM 92-20-04	Box of 96 PhyTip columns (200 µL volume) 20 µL of Glutathione affinity resin per column
Glutathione	Tecan Freedom Evo MCA 96-well	500	96	20	PTM 95-20-04	Box of 96 PhyTip columns (500 µL volume) 20 µL of Glutathione affinity resin per column
Glutathione	Tecan Freedom Evo MCA 96-well	500	96	40	PTM 95-40-04	Box of 96 PhyTip columns (500 µL volume) 40 µL of Glutathione affinity resin per column
Glutathione	Tecan Freedom Evo MCA 96-well	500	96	80	PTM 95-80-04	Box of 96 PhyTip columns (500 µL volume) 80 µL of Glutathione affinity resin per column
Glutathione	Tecan Freedom Evo MCA 96-well	500	96	160	PTM 95-16-04	Box of 96 PhyTip columns (500 µL volume) 160 µL of Glutathione affinity resin per column

Robotic System:

Tip Volume:

Resin Volume:

Resin Robotic System Tip Volume QTY/Box Resin Volume Part Number Description

Glutathione Agilent Velocity 11 Bravo & V-Prep 200 96 20 PTV 92-20-04 Box of 96 PhyTip columns (200 µL volume) 20 µL of Glutathione affinity resin per column

Glutathione Beckman FX & NX 96-well 200 96 5 PTB 92-05-04 Box of 96 PhyTip columns (200 µL volume) 5 µL of Glutathione affinity resin per column

Glutathione Beckman FX & NX 96-well 200 96 20 PTB 92-20-04 Box of 96 PhyTip columns (200 µL volume) 20 µL of Glutathione affinity resin per column

Glutathione Caliper SciClone & Zephyr 96 channel_now Perkin Elmer 200 96 5 PTZ 92-05-04 Box of 96 PhyTip columns (200 µL volume) 5 µL of Glutathione affinity resin per column

Glutathione Caliper SciClone & Zephyr 96 channel_now Perkin Elmer 200 96 20 PTZ 92-20-04 Box of 96 PhyTip columns (200 µL volume) 20 µL of Glutathione affinity resin per column

Glutathione Dynamic Devices Oasis & Lynx 1000 96 10 PTD 91-10-04 Box of 96 PhyTip columns (1 mL volume) 10 µL of Glutathione affinity resin per column

Glutathione Dynamic Devices Oasis & Lynx 1000 96 20 PTD 91-20-04 Box of 96 PhyTip columns (1 mL volume) 20 µL of Glutathione affinity resin per column

Glutathione Dynamic Devices Oasis & Lynx 1000 96 40 PTD 91-40-04 Box of 96 PhyTip columns (1 mL volume) 40 µL of Glutathione affinity resin per column

Glutathione Dynamic Devices Oasis & Lynx 1000 96 80 PTD 91-80-04 Box of 96 PhyTip columns (1 mL volume) 80 µL of Glutathione affinity resin per column

Glutathione Dynamic Devices Oasis & Lynx 1000 96 160 PTD 91-16-04 Box of 96 PhyTip columns (1 mL volume) 160 µL of Glutathione affinity resin per column

Glutathione Dynamic Devices Oasis & Lynx 200 96 5 PTD 92-05-04 Box of 96 PhyTip columns (200 µL volume) 5 µL of Glutathione affinity resin per column

Glutathione Dynamic Devices Oasis & Lynx 200 96 20 PTD 92-20-04 Box of 96 PhyTip columns (200 µL volume) 20 µL of Glutathione affinity resin per column

Glutathione Hamilton STAR & Nimbus 1000 96 10 PTH 91-10-04 Box of 96 PhyTip columns (1 mL volume) 10 µL of Glutathione affinity resin per column

Glutathione Hamilton STAR & Nimbus 1000 96 20 PTH 91-20-04 Box of 96 PhyTip columns (1 mL volume) 20 µL of Glutathione affinity resin per column

Glutathione Hamilton STAR & Nimbus 1000 96 40 PTH 91-40-04 Box of 96 PhyTip columns (1 mL volume) 40 µL of Glutathione affinity resin per column

Glutathione Hamilton STAR & Nimbus 1000 96 80 PTH 91-80-04 Box of 96 PhyTip columns (1 mL volume) 80 µL of Glutathione affinity resin per column

Glutathione Hamilton STAR & Nimbus 1000 96 160 PTH 91-16-04 Box of 96 PhyTip columns (1 mL volume) 160 µL of Glutathione affinity resin per column

Glutathione Hamilton STAR & Nimbus 300 96 5 PTH 93-05-04 Box of 96 PhyTip columns (300 µL volume) 5 µL of Glutathione affinity resin per column

Glutathione Hamilton STAR & Nimbus 300 96 20 PTH 93-20-04 Box of 96 PhyTip columns (300 µL volume) 20 µL of Glutathione affinity resin per column

Glutathione Perkin Elmer Janus Varispan 4 or 8-well 1000 96 10 PTJ 91-10-04 Box of 96 PhyTip columns (1 mL volume) 10 µL of Glutathione affinity resin per column

Glutathione Perkin Elmer Janus Varispan 4 or 8-well 1000 96 20 PTJ 91-20-04 Box of 96 PhyTip columns (1 mL volume) 20 µL of Glutathione affinity resin per column

Glutathione Perkin Elmer Janus Varispan 4 or 8-well 1000 96 40 PTJ 91-40-04 Box of 96 PhyTip columns (1 mL volume) 40 µL of Glutathione affinity resin per column

Glutathione Perkin Elmer Janus Varispan 4 or 8-well 1000 96 80 PTJ 91-80-04 Box of 96 PhyTip columns (1 mL volume) 80 µL of Glutathione affinity resin per column

Glutathione Perkin Elmer Janus Varispan 4 or 8-well 1000 96 160 PTJ 91-16-04 Box of 96 PhyTip columns (1 mL volume) 160 µL of Glutathione affinity resin per column

Glutathione Perkin Elmer Packard MiniTrack & Janus MDT 96 channel 200 96 5 PTP 92-05-04 Box of 96 PhyTip columns (200 µL volume) 5 µL of Glutathione affinity resin per column

Glutathione Perkin Elmer Packard MiniTrack & Janus MDT 96 channel 200 96 20 PTP 92-20-04 Box of 96 PhyTip columns (200 µL volume) 20 µL of Glutathione affinity resin per column

Glutathione PhyNexus MEA or ME 1000 96 10 PTR 91-10-04 Box of 96 PhyTip columns (1 mL volume) 10 µL of Glutathione affinity resin per column

Glutathione PhyNexus MEA or ME 1000 96 20 PTR 91-20-04 Box of 96 PhyTip columns (1 mL volume) 20 µL of Glutathione affinity resin per column

Glutathione PhyNexus MEA or ME 1000 96 40 PTR 91-40-04 Box of 96 PhyTip columns (1 mL volume) 40 µL of Glutathione affinity resin per column

Glutathione PhyNexus MEA or ME 1000 96 80 PTR 91-80-04 Box of 96 PhyTip columns (1 mL volume) 80 µL of Glutathione affinity resin per column

Glutathione PhyNexus MEA or ME 1000 96 160 PTR 91-16-04 Box of 96 PhyTip columns (1 mL volume) 160 µL of Glutathione affinity resin per column

Glutathione PhyNexus MEA or ME 1000 96 320 PTR 91-32-04 Box of 96 PhyTip columns (1 mL volume) 320 µL of Glutathione affinity resin per column

Glutathione PhyNexus MEA or ME 200 96 5 PTR 92-05-04 Box of 96 PhyTip columns (200 µL volume) 5 µL of Glutathione affinity resin per column

Glutathione PhyNexus MEA or ME 200 96 20 PTR 92-20-04 Box of 96 PhyTip columns (200 µL volume) 20 µL of Glutathione affinity resin per column

Glutathione Tecan Freedom Evo LiHa & Tecan Te-MO 1000 96 10 PTT 91-10-04 Box of 96 PhyTip columns (1 mL volume) 10 µL of Glutathione affinity resin per column

Glutathione Tecan Freedom Evo LiHa & Tecan Te-MO 1000 96 20 PTT 91-20-04 Box of 96 PhyTip columns (1 mL volume) 20 µL of Glutathione affinity resin per column

Glutathione Tecan Freedom Evo LiHa & Tecan Te-MO 1000 96 40 PTT 91-40-04 Box of 96 PhyTip columns (1 mL volume) 40 µL of Glutathione affinity resin per column

Glutathione Tecan Freedom Evo LiHa & Tecan Te-MO 1000 96 80 PTT 91-80-04 Box of 96 PhyTip columns (1 mL volume) 80 µL of Glutathione affinity resin per column

Glutathione Tecan Freedom Evo LiHa & Tecan Te-MO 1000 96 160 PTT 91-16-04 Box of 96 PhyTip columns (1 mL volume) 160 µL of Glutathione affinity resin per column

Glutathione Tecan Freedom Evo LiHa & Tecan Te-MO 200 96 5 PTT 92-05-04 Box of 96 PhyTip columns (200 µL volume) containing 5 µL of Glutathione affinity resin per column

Glutathione Tecan Freedom Evo LiHa & Tecan Te-MO 200 96 20 PTT 92-20-04 Box of 96 PhyTip columns (200 µL volume) containing 20 µL of Glutathione affinity resin per column

Glutathione Tecan Freedom Evo MCA 96-well 200 96 5 PTM 92-05-04 Box of 96 PhyTip columns (200 µL volume) 5 µL of Glutathione affinity resin per column

Glutathione Tecan Freedom Evo MCA 96-well 200 96 20 PTM 92-20-04 Box of 96 PhyTip columns (200 µL volume) 20 µL of Glutathione affinity resin per column

Glutathione Tecan Freedom Evo MCA 96-well 500 96 20 PTM 95-20-04 Box of 96 PhyTip columns (500 µL volume) 20 µL of Glutathione affinity resin per column

Glutathione Tecan Freedom Evo MCA 96-well 500 96 40 PTM 95-40-04 Box of 96 PhyTip columns (500 µL volume) 40 µL of Glutathione affinity resin per column

Glutathione Tecan Freedom Evo MCA 96-well 500 96 80 PTM 95-80-04 Box of 96 PhyTip columns (500 µL volume) 80 µL of Glutathione affinity resin per column

Glutathione Tecan Freedom Evo MCA 96-well 500 96 160 PTM 95-16-04 Box of 96 PhyTip columns (500 µL volume) 160 µL of Glutathione affinity resin per column

Glutathione Columns

Glutathione PhyTip Columns

Products

Products Catalogs

Applications

PhyTip Columns for Robots

Technology

About PhyNexus

GST-tagged Proteins – Glutathione Columns

Glutathione Columns

Glutathione PhyTip Columns

Products

Products Catalogs

Applications

PhyTip Columns for Robots

Technology

About PhyNexus