Normal Phase Chromatography Columns

PhyTip^® Normal Phase Resin columns are designed for the specific enrichment of polar compounds. These PhyTip columns are especially useful in oligosaccharide mapping and identification analysis of glycoproteins. High resolution tools such as HPLC and capillary electrophoresis are required for the separations of these complicated structures; however, detection must be enhanced through the use of fluorescent dye labels. PhyTip Normal Phase Resin columns are the perfect solution for high throughput, reproducible sample dye removal prior to identification and quantification.

PhyTip normal phase columns function to separate and enrich polar compounds such as oligosaccharides.
They are the only high throughput, automatable, oligosaccharide purification solution commercially available.
Similar to the PhyTip affinity columns, the resin of choice is positioned between two inert screens that maintain the resin bed at the base of the column.
An excellent solution for excess dye removal prior to CE or HPLC analysis of oligosaccharides.

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Description

PhyTip Normal Phase 1 columns PhyTip

Normal Phase 1 columns have been optimized for use with specific PhyNexus reagents and instrument flow rates/volumes as shown below. This information was collected using the MEA Personal Purification System.

These columns are ideal for the purification of fluorescently-labeled glycans required the removal of excess dye. PhyNexus recommends using the following buffers:

Conditioning Solution – 20% Acetonitrile

Equilibration Solution – 95% Acetonitrile

Capture Solution – Dilute 200μL samples five fold by adding 800μL 95% acetonitrile.

Wash Solution – 95% Acetonitrile

Elution Solution – 20% Acetonitrile

Application

PhyTip columns are unique capture, purification and enrichment tools from PhyNexus designed for small volume protein sample preparation. PhyTip Normal Phase 1 columns are designed for the purification of glycans, oligosaccharides and complex carbohydrates. PhyTip columns are available for a variety of liquid handling platforms and contain specific affinity resins for application specific requirements. Samples for purification and enrichment must be clear and free from particulate matter. It is highly recommended to centrifuge samples and use the clear supernatant only, prior to use with PhyTip columns.

Data

PhyTip Columns

PhyTip columns are available in two formats, 200+ with a recommended maximum sample volume of 200 μL and 1000+ with a recommended maximum volume of 1000 μL. For each of the PhyTip column formats there are a number of different resin volumes available. Each PhyTip column has been designed for maximum efficiency of capture and elution of the specific protein(s) of interest when using the specified protocol.

Shipping and Storage

Each pack of PhyTip columns has been manufactured and QC’d to the highest standards and shipped in retainer boxes that maintain the integrity of the specific affinity resin within each PhyTip column. This product is shipped at ambient temperatures, but on receipt should be stored in a standard laboratory refrigerator between 4 and 8◦C.

Do NOT freeze or store frozen.
When not in use, keep the lid of the box closed and sealed, store in the refrigerator.
PhyTip Normal Phase 1 columns are NOT stored with additives when shipped from PhyNexus.

Documents

Normal Phase Product Insert Sheet

Normal Phase Glycoprotein Technical Note

Normal Phase 1 PhyTip Column MSDS

Robotics Systems Compatibility

Glycan Clean-up and Custom Oligosaccharide Columns are available for these robotic systems.

Robotic Systems
Agilent Velocity 11 Bravo & V-Prep
Beckman FX & NX 96-well
Caliper SciClone & Zephyr 96-channel (now Perkin Elmer)
Dynamic Devices Oasis & Lynx
Hamilton STAR & Nimbus
Perkin Elmer Janus Varispan 4 or 8-well
Perkin Elmer Packard MiniTrack & Janus MDT 96-channel
PhyNexus MEA or ME
Tecan Freedom Evo LiHa & Tecan Te-MO
Tecan Freedom Evo MCA 96-well

Part Numbers

Normal Phase 1 PhyTip Columns

Resin	Robotic System	Tip Volume	QTY/Box	Resin Volume	Part Number	Description
Normal Phase 1	Agilent Velocity 11 Bravo & V-Prep	200	96	20	PTV 92-20-09	Box of 96 PhyTip columns (200 µL volume) 20 µL of Normal Phase I resin per column
Normal Phase 1	Beckman FX & NX 96-well	200	96	5	PTB 92-05-09	Box of 96 PhyTip columns (200 µL volume) 5 µL of Normal Phase I resin per column
Normal Phase 1	Beckman FX & NX 96-well	200	96	20	PTB 92-20-09	Box of 96 PhyTip columns (200 µL volume) 20 µL of Normal Phase I resin per column
Normal Phase 1	Caliper SciClone & Zephyr 96 channel_now Perkin Elmer	200	96	5	PTZ 92-05-09	Box of 96 PhyTip columns (200 µL volume) 5 µL of Normal Phase I resin per column
Normal Phase 1	Caliper SciClone & Zephyr 96 channel_now Perkin Elmer	200	96	20	PTZ 92-20-09	Box of 96 PhyTip columns (200 µL volume) 20 µL of Normal Phase I resin per column
Normal Phase 1	Dynamic Devices Oasis & Lynx	1000	96	10	PTD 91-10-09	Box of 96 PhyTip columns (1 mL volume) 10 µL of Normal Phase I affinity resin per column
Normal Phase 1	Dynamic Devices Oasis & Lynx	1000	96	20	PTD 91-20-09	Box of 96 PhyTip columns (1 mL volume) 20 µL of Normal Phase I affinity resin per column
Normal Phase 1	Dynamic Devices Oasis & Lynx	200	96	5	PTD 92-05-09	Box of 96 PhyTip columns (200 µL volume) 5 µL of Normal Phase I affinity resin per column
Normal Phase 1	Dynamic Devices Oasis & Lynx	200	96	20	PTD 92-20-09	Box of 96 PhyTip columns (200 µL volume) 20 µL of Normal Phase I affinity resin per column
Normal Phase 1	Hamilton STAR & Nimbus	1000	96	10	PTH 91-10-09	Box of 96 PhyTip columns (1 mL volume) 10 µL of Normal Phase I resin per column
Normal Phase 1	Hamilton STAR & Nimbus	1000	96	20	PTH 91-20-09	Box of 96 PhyTip columns (1 mL volume) 20 µL of Normal Phase I resin per column
Normal Phase 1	Hamilton STAR & Nimbus	300	96	5	PTH 93-05-09	Box of 96 PhyTip columns (300 µL volume) 5 µL of Normal Phase I resin per column
Normal Phase 1	Hamilton STAR & Nimbus	300	96	20	PTH 93-20-09	Box of 96 PhyTip columns (300 µL volume) 20 µL of Normal Phase I resin per column
Normal Phase 1	Perkin Elmer Janus Varispan 4 or 8-well	1000	96	10	PTJ 91-10-09	Box of 96 PhyTip columns (1 mL volume) 10 µL of Normal Phase I affinity resin per column
Normal Phase 1	Perkin Elmer Janus Varispan 4 or 8-well	1000	96	20	PTJ 91-20-09	Box of 96 PhyTip columns (1 mL volume) 20 µL of Normal Phase I affinity resin per column
Normal Phase 1	Perkin Elmer Packard MiniTrack & Janus MDT 96 channel	200	96	5	PTP 92-05-09	Box of 96 PhyTip columns (200 µL volume) 5 µL of Normal Phase I resin per column
Normal Phase 1	Perkin Elmer Packard MiniTrack & Janus MDT 96 channel	200	96	20	PTP 92-20-09	Box of 96 PhyTip columns( 200 µL volume) 20 µL of Normal Phase I resin per column
Normal Phase 1	PhyNexus MEA or ME	1000	96	10	PTR 91-10-09	Box of 96 PhyTip columns (1 mL volume) 10 µL of Normal Phase I resin per column
Normal Phase 1	PhyNexus MEA or ME	1000	96	20	PTR 91-20-09	Box of 96 PhyTip columns (1 mL volume) 20 µL of Normal Phase I resin per column
Normal Phase 1	PhyNexus MEA or ME	200	96	5	PTR 92-05-09	Box of 96 PhyTip columns( 200 µL volume) 5 µL of Normal Phase I resin per column
Normal Phase 1	PhyNexus MEA or ME	200	96	20	PTR 92-20-09	Box of 96 PhyTip columns( 200 µL volume) 20 µL of Normal Phase I resin per column
Normal Phase 1	Tecan Freedom Evo LiHa & Tecan Te-MO	200	96	5	PTT 92-05-09	Box of 96 PhyTip columns (200 µL volume) containing 5 µL of Normal Phase I resin per column
Normal Phase 1	Tecan Freedom Evo LiHa & Tecan Te-MO	200	96	20	PTT 92-20-09	Box of 96 PhyTip columns (200 µL volume) containing 20 µL of Normal Phase I resin per column
Normal Phase 1	Tecan Freedom Evo MCA 96-well	200	96	5	PTM 92-05-09	Box of 96 PhyTip columns (200 µL volume) 5 µL of Normal Phase I resin per column
Normal Phase 1	Tecan Freedom Evo MCA 96-well	200	96	20	PTM 92-20-09	Box of 96 PhyTip columns (200 µL volume) 20 µL of Normal Phase I resin per column
Normal Phase 1	Tecan Freedom Evo MCA 96-well	500	96	20	PTM 95-20-09	Box of 96 PhyTip columns (500 µL volume) 20 µL of Normal Phase I resin per column
Normal Phase 1	Tecan Freedom Evo MCA 96-well	500	96	40	PTM 95-40-09	Box of 96 PhyTip columns (500 µL volume) 40 µL of Normal Phase I resin per column
Normal Phase 1	Tecan Freedom Evo MCA 96-well	500	96	80	PTM 95-80-09	Box of 96 PhyTip columns (500 µL volume) 80 µL of Normal Phase I resin per column
Normal Phase 1	Tecan Freedom Evo MCA 96-well	500	96	160	PTM 95-16-09	Box of 96 PhyTip columns (500 µL volume) 160 µL of Normal Phase I resin per column

Robotic System:

Tip Volume:

Resin Volume:

Resin Robotic System Tip Volume QTY/Box Resin Volume Part Number Description

Normal Phase 1 Agilent Velocity 11 Bravo & V-Prep 200 96 20 PTV 92-20-09 Box of 96 PhyTip columns (200 µL volume) 20 µL of Normal Phase I resin per column

Normal Phase 1 Beckman FX & NX 96-well 200 96 5 PTB 92-05-09 Box of 96 PhyTip columns (200 µL volume) 5 µL of Normal Phase I resin per column

Normal Phase 1 Beckman FX & NX 96-well 200 96 20 PTB 92-20-09 Box of 96 PhyTip columns (200 µL volume) 20 µL of Normal Phase I resin per column

Normal Phase 1 Caliper SciClone & Zephyr 96 channel_now Perkin Elmer 200 96 5 PTZ 92-05-09 Box of 96 PhyTip columns (200 µL volume) 5 µL of Normal Phase I resin per column

Normal Phase 1 Caliper SciClone & Zephyr 96 channel_now Perkin Elmer 200 96 20 PTZ 92-20-09 Box of 96 PhyTip columns (200 µL volume) 20 µL of Normal Phase I resin per column

Normal Phase 1 Dynamic Devices Oasis & Lynx 1000 96 10 PTD 91-10-09 Box of 96 PhyTip columns (1 mL volume) 10 µL of Normal Phase I affinity resin per column

Normal Phase 1 Dynamic Devices Oasis & Lynx 1000 96 20 PTD 91-20-09 Box of 96 PhyTip columns (1 mL volume) 20 µL of Normal Phase I affinity resin per column

Normal Phase 1 Dynamic Devices Oasis & Lynx 200 96 5 PTD 92-05-09 Box of 96 PhyTip columns (200 µL volume) 5 µL of Normal Phase I affinity resin per column

Normal Phase 1 Dynamic Devices Oasis & Lynx 200 96 20 PTD 92-20-09 Box of 96 PhyTip columns (200 µL volume) 20 µL of Normal Phase I affinity resin per column

Normal Phase 1 Hamilton STAR & Nimbus 1000 96 10 PTH 91-10-09 Box of 96 PhyTip columns (1 mL volume) 10 µL of Normal Phase I resin per column

Normal Phase 1 Hamilton STAR & Nimbus 1000 96 20 PTH 91-20-09 Box of 96 PhyTip columns (1 mL volume) 20 µL of Normal Phase I resin per column

Normal Phase 1 Hamilton STAR & Nimbus 300 96 5 PTH 93-05-09 Box of 96 PhyTip columns (300 µL volume) 5 µL of Normal Phase I resin per column

Normal Phase 1 Hamilton STAR & Nimbus 300 96 20 PTH 93-20-09 Box of 96 PhyTip columns (300 µL volume) 20 µL of Normal Phase I resin per column

Normal Phase 1 Perkin Elmer Janus Varispan 4 or 8-well 1000 96 10 PTJ 91-10-09 Box of 96 PhyTip columns (1 mL volume) 10 µL of Normal Phase I affinity resin per column

Normal Phase 1 Perkin Elmer Janus Varispan 4 or 8-well 1000 96 20 PTJ 91-20-09 Box of 96 PhyTip columns (1 mL volume) 20 µL of Normal Phase I affinity resin per column

Normal Phase 1 Perkin Elmer Packard MiniTrack & Janus MDT 96 channel 200 96 5 PTP 92-05-09 Box of 96 PhyTip columns (200 µL volume) 5 µL of Normal Phase I resin per column

Normal Phase 1 Perkin Elmer Packard MiniTrack & Janus MDT 96 channel 200 96 20 PTP 92-20-09 Box of 96 PhyTip columns( 200 µL volume) 20 µL of Normal Phase I resin per column

Normal Phase 1 PhyNexus MEA or ME 1000 96 10 PTR 91-10-09 Box of 96 PhyTip columns (1 mL volume) 10 µL of Normal Phase I resin per column

Normal Phase 1 PhyNexus MEA or ME 1000 96 20 PTR 91-20-09 Box of 96 PhyTip columns (1 mL volume) 20 µL of Normal Phase I resin per column

Normal Phase 1 PhyNexus MEA or ME 200 96 5 PTR 92-05-09 Box of 96 PhyTip columns( 200 µL volume) 5 µL of Normal Phase I resin per column

Normal Phase 1 PhyNexus MEA or ME 200 96 20 PTR 92-20-09 Box of 96 PhyTip columns( 200 µL volume) 20 µL of Normal Phase I resin per column

Normal Phase 1 Tecan Freedom Evo LiHa & Tecan Te-MO 200 96 5 PTT 92-05-09 Box of 96 PhyTip columns (200 µL volume) containing 5 µL of Normal Phase I resin per column

Normal Phase 1 Tecan Freedom Evo LiHa & Tecan Te-MO 200 96 20 PTT 92-20-09 Box of 96 PhyTip columns (200 µL volume) containing 20 µL of Normal Phase I resin per column

Normal Phase 1 Tecan Freedom Evo MCA 96-well 200 96 5 PTM 92-05-09 Box of 96 PhyTip columns (200 µL volume) 5 µL of Normal Phase I resin per column

Normal Phase 1 Tecan Freedom Evo MCA 96-well 200 96 20 PTM 92-20-09 Box of 96 PhyTip columns (200 µL volume) 20 µL of Normal Phase I resin per column

Normal Phase 1 Tecan Freedom Evo MCA 96-well 500 96 20 PTM 95-20-09 Box of 96 PhyTip columns (500 µL volume) 20 µL of Normal Phase I resin per column

Normal Phase 1 Tecan Freedom Evo MCA 96-well 500 96 40 PTM 95-40-09 Box of 96 PhyTip columns (500 µL volume) 40 µL of Normal Phase I resin per column

Normal Phase 1 Tecan Freedom Evo MCA 96-well 500 96 80 PTM 95-80-09 Box of 96 PhyTip columns (500 µL volume) 80 µL of Normal Phase I resin per column

Normal Phase 1 Tecan Freedom Evo MCA 96-well 500 96 160 PTM 95-16-09 Box of 96 PhyTip columns (500 µL volume) 160 µL of Normal Phase I resin per column

Normal Phase 1 PhyTip Columns

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PhyTip Columns for Robots

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About PhyNexus

Normal Phase PhyTip Columns

Normal Phase 1 PhyTip Columns

Products

Products Catalogs

Applications

PhyTip Columns for Robots

Technology

About PhyNexus