PhyTip® Normal Phase Resin columns are designed for the specific enrichment of polar compounds. These PhyTip columns are especially useful in oligosaccharide mapping and identification analysis of glycoproteins. High resolution tools such as HPLC and capillary electrophoresis are required for the separations of these complicated structures; however, detection must be enhanced through the use of fluorescent dye labels. PhyTip Normal Phase Resin columns are the perfect solution for high throughput, reproducible sample dye removal prior to identification and quantification.

  • Normal phase tips function to separate and enrich polar compounds such as oligosaccharides.
  • They are the only high throughput, automatable, oligosaccharide purification solution commercially available.
  • Similar to the PhyTip affinity columns, the resin of choice is positioned between two inert screens that maintain the resin bed at the base of the column.
  • An excellent solution for excess dye removal prior to CE or HPLC analysis of oligosaccharides.

Normal Phase Resin columns with our proprietary Dual Flow Chromatography provide complete antibody binding and high final concentrations. PhyTip columns are compatible with most 8, 12, and 96-channel automation liquid handling robots including Agilent TechnologiesBeckman CoulterDynamic DevicesHamilton, Opentrons, Perkin Elmer, and Tecan.

PhyNexus PhyTip Normal Phase

Normal Phase Resin tips are available in tip volumes (µL) of 200|300|500|1000 and resin volumes (µL) of 5|10|20|40|80|160|320 depending upon robot configuration.

PhyTip Normal Phase 1 columns PhyTip

Normal Phase 1 columns have been optimized for use with specific PhyNexus reagents and instrument flow rates/volumes as shown below. This information was collected using the MEA Personal Purification System.

These columns are ideal for the purification of fluorescently-labeled glycans required the removal of excess dye. PhyNexus recommends using the following buffers:

Conditioning Solution – 20% Acetonitrile

Equilibration Solution – 95% Acetonitrile

Capture Solution – Dilute 200μL samples five fold by adding 800μL 95% acetonitrile.

Wash Solution – 95% Acetonitrile

Elution Solution – 20% Acetonitrile

Select your resin, robotic system, tip and resin volume and see the results in the table below.

Normal Phase Columns