PhyTip® Ion Exchange columns offer the unique ability to screen different wash and elution buffers and a number of resins in a single experiment. Once ion exchange conditions have been optimized, they are directly transferable to larger manufacturing scale columns. Chromatographic separation is performed in a pipette tip column using back and forth flow to drive binding and release steps to equilibrium. Using the MEA 2 Personal Purification System enables 12 samples at a time to be processed. This can be increased to 96 samples with the use of robotic liquid handling systems. Four standard ion exchange resins are available in PhyTip columns (strong anion, weak anion, strong cation, weak cation). For resin selection, the Ion Exchange Resin Screening Toolkit is a box of 96 tips containing 24 columns of each resin.

One advantage to ion exchange is the buffer conditions used for capture washing and elution are generally compatible with the proteins so that high activity can be retained in the final product. Moreover, ion exchange chromatography may still be used for final purification and polishing. Using ion exchange as an orthogonal chemistry to affinity chromatography can result in very pure proteins.

There are two basic types of ion-exchangers: cation exchangers and anion exchangers. Cation exchange separations are performed at pH’s between 4 – 7 and anion exchange separations are generally done at pH’s between 5 – 8.

Within anion exchange there two types: strong base anion exchange and weak base anion exchange. The same is true for cation exchange: strong acid cation exchange and weak acid cation exchange.

Choosing the most effective ion exchanger and ion exchange conditions ion exchange purification may be difficult to determine. The PhyNexus PhyTip column technology based on pipette tip columns can easily process samples in parallel making it effective to screen dozens to hundreds of ion exchange within a very short time.

Description

PhyNexus’ family of PhyTip products includes ion exchange. In traditional ion exchange chromatography, samples, wash buffers and gradient elution buffers pass through packed columns in one direction. The PhyTip column is able to simulate this chromatographic separation in a small, pipette tip column by driving the binding and release steps to equilibrium. This is not achieved by using high back-pressure, large columns, but instead by PhyNexus’ patented back and-forth flow.

  • Develop ion exchange purification methods in a fast, efficient manner
  • Automation compatible on the MEA Personal Purification System or other robotic systems
  • Take advantage of parallel processing to screen resins
  • Applications include conditions optimization, imidazole removal after His-tagged protein purification and high-throughput replacement of finishing columns
Data

PhyTip ion exchange columns available as a custom product, for ordering details please contact PhyNexus Columns are available in the following sizes: 200 μL column with ion exchange resin volume of 5 μL and 20 μL 1000 μL column with Pro Plus resin volume of 10, 20, 40, 80, 160, and 320μL.

Documents
Robotics Systems Compatibility

Ion Exchange PhyTip Columns are available for these robotic systems.

Part Numbers

Ion Exchange PhyTip Columns