Trypsin is a serine protease and is the most commonly used enzyme for the digestion of proteins used in mass spectrometry based proteomics and analysis. Trypsin cleaves peptide bonds at the C-terminal side of positively charged Lysine(Lys) and Arginine(Arg) amino acid residues which is advantageous for mass spectrometry analysis. After digestion, LC-MS MS or MALDI-TOF are used for peptide fingerprinting.
Standard tryptic digestion can take up to 1.5 days including the overnight trypsin digestion and is time-consuming and labor intensive. The Biotage immobilized trypsin PhyTip columns provide a fast automated way for on-column digestion of simple or complex protein mixtures. The Biotage workflow includes denaturing, reduction, alkylation, tryptic digestion, and desalting with the Biotage C18 columns.
- Fully automated trypsin digestion in 1.5 hours including desalting
- 1-96 samples at a time
- No heat block, incubation, centrifugation, or filtration required
- High concentrations of proteins can be used
- Suitable for many application including protein identification, Quantitative analysis, protein therapeutics characterization, protein post-translational modifications, protein sequencing, and protein to protein interactions
The Biotage immobilized trypsin PhyTip columns with our proprietary Dual Flow Chromatography provide a complete and fully automated, high throughput workflow for mass spectroscopy analysis. Trypsin PhyTip columns are compatible with most 8, 12, and 96-channel automation liquid handling robots including MEA2, Agilent Technologies, Beckman Coulter, Dynamic Devices, Hamilton, Opentrons, Perkin Elmer, and Tecan.
Immobilized Trypsin tips are available in 200 µL and 300 µL tip volumes (depending upon robot configuration) with resin volume of 5 µL.