Standard tryptic digestion can take up to 1.5 days including the overnight trypsin digestion and is time-consuming and labor intensive. The Biotage immobilized trypsin PhyTip columns provide a fast automated way for on-column digestion of simple or complex protein mixtures. The Biotage workflow includes denaturing, reduction, alkylation, tryptic digestion, and desalting with the Biotage C18 columns.
- Fully automated trypsin digestion in 1.5 hours including desalting
- 1-96 samples at a time
- No heat block, incubation, centrifugation, or filtration required
- High concentrations of proteins can be used
- Suitable for many application including protein identification, Quantitative analysis, protein therapeutics characterization, protein post-translational modifications, protein sequencing, and protein to protein interactions