Trypsin is a serine protease and is the most commonly used enzyme for the digestion of proteins used in mass spectrometry based proteomics and analysis. Trypsin cleaves peptide bonds at the C-terminal side of positively charged Lysine(Lys) and Arginine(Arg) amino acid residues which is advantageous for mass spectrometry analysis. After digestion, LC-MS MS or MALDI-TOF are used for peptide fingerprinting.

Biotage-Fully automated-Trypsin-Digestion-columns

Standard tryptic digestion can take up to 1.5 days including the overnight trypsin digestion and is time-consuming and labor intensive. The Biotage immobilized trypsin PhyTip columns provide a fast automated way for on-column digestion of simple or complex protein mixtures. The Biotage workflow includes denaturing, reduction, alkylation, tryptic digestion, and desalting with the Biotage C18 columns.

  • Fully automated trypsin digestion in 1.5 hours including desalting
  • 1-96 samples at a time
  • No heat block, incubation, centrifugation, or filtration required
  • High concentrations of proteins can be used
  • Suitable for many application including protein identification, Quantitative analysis, protein therapeutics characterization, protein post-translational modifications, protein sequencing, and protein to protein interactions
Biotage-fully-automated-Trypsin-Digestion-columns-workflow

The Biotage immobilized trypsin PhyTip columns with our proprietary Dual Flow Chromatography provide a complete and fully automated, high throughput workflow for mass spectroscopy analysis. Trypsin PhyTip columns are compatible with most 8, 12, and 96-channel automation liquid handling robots including MEA2, Agilent TechnologiesBeckman CoulterDynamic DevicesHamilton, Opentrons, Perkin Elmer, and Tecan.