PhyNexus has developed an automated process to desalt or “clean-up” proteins/peptides using C18 columns and C4 columns in order to streamline sample preparation for mass spectrometry analysis (up to 96 at a time). When preforming a reversed phase purification using a manual pipette, back pressure in aspirating solutions can cause an inconvenience to the operator and inconsistencies within results. PhyTip columns perform the clean-up with the consistency, ease, and scalability that go hand-in-hand with automation. With the high capacity and versatility in protein binding, the PhyNexus C18 column is the optimal choice in developing a fluid MALDI or LC-MS workflow.
C18 columns have increased selectivity for polar and non-polar molecules compared to other columns due to the increased bed size and capacity of the PhyTip pipette tip column. This larger bed size will push the equilibrium of the capture to increased amount of capture product. When comparing the capacity of some of the leading C18 tips, the C18 PhyTip column binds more than double the amount of protein per mL of resin while exhibiting a comparable recovery and range.
C18 columns are more hydrophobic than C4 columns providing outstanding sensitivity and high efficiency binding of peptides. Both medium 90 Å and large 300 Å pores are available for differential selectivity of peptides and protein fragments. When choosing between C4 and C18 resin, the major difference is that C4 is slightly more polar than C18 due to its shorter length and closer proximity to the polar silica substrate. Therefore, the selectivity of the C4 resin is increased for more polar molecules.
The columns bind a wide range of protein and polypeptide sizes. The absolute binding capacity is high with a low loss of smaller or more polar polypeptides. The sample is recovered in common mass spectrometry compatible solvents. In addition, gel filtration columns may be used to perform rapid and completely automated 96 at-a-time buffer desalting or buffer exchange.
C18 tips with our proprietary Dual Flow Chromatography provide complete antibody binding and high final concentrations. C18 PhyTip columns are compatible with most 8, 12, and 96-channel automation liquid handling robots including Agilent Technologies, Beckman Coulter, Dynamic Devices, Hamilton, Opentrons, Perkin Elmer, and Tecan.
Related: Normal Phase Ion Exchange Mass Spectrometry C4
C18 tips are available in tip volumes (µL) of 200|300|500|1000 and resin volumes (µL) of 5|10|20|40|80|160|320 depending upon robot configuration.
PhyNexus has developed an automated process to desalt or “clean-up” proteins/peptides using C18 PhyTip® columns for use in mass spectrometry (MS) analysis. PhyTip columns bind proteins/peptides through reversed-phase solid phase extraction (SPE), which utilizes long carbon chain C18 resins to capture and separate peptides based on their hydrophobicity.
The PhyTip column is composed of C18 resins retained on a pipette tip by two thin, inert frit screens. Depending on the downstream assay, the PhyTip column can be made with either 90 Å or 300 Å pore resin in a 5 µL bed and has capacity to process up to 200 µL of solution. The PhyTip column processes peptides through dual flow chromatography, a systematic process of back and forth sample flow performed by a liquid handler that controls the volume and flow rates.
As a result, the method can be utilized to clean-up small to mid-scale peptide samples (up to 96 at a time) for use in matrix-assisted laser desorption-ionization (MALDI) or electrospray ionization (ESI) mass spectrometry as part of an automated process.
Mass Spectrometry PhyTip Columns are available for these robotic systems.
| Robotic Systems |
|---|
| Agilent Velocity 11 Bravo & V-Prep |
| Beckman FX & NX 96-well |
| Caliper SciClone & Zephyr 96-channel (now Perkin Elmer) |
| Dynamic Devices Oasis & Lynx |
| Hamilton STAR & Nimbus |
| Perkin Elmer Janus Varispan 4 or 8-well |
| Perkin Elmer Packard MiniTrack & Janus MDT 96-channel |
| PhyNexus MEA or ME |
| Tecan Freedom Evo LiHa & Tecan Te-MO |
| Tecan Freedom Evo MCA 96-well |
Posters
New Advances towards Quantitative Proteomics: Optimization of Peptide Labeling by Fluorescent Isotope Coded Affinity Tag (FCAT)
Z. Rivera, M. Olajos, T. Ringer, A.R. Mayer, G. K. Bonn and A. Guttman (Horvath Laboratory of Bioseparation Sciences, Austria)
A flexible platform for automated high-performance protein purification using micro scale separation technology
J. Lambert, M. Anderson, D. Gjerde, L. Nguyen (PhyNexus)
Innovative Solutions for Automated Protein and Nucleic Acid Purification
Lee Hoang, PhyNexus, Inc., PhyNexus Webinar
Automated, High Throughput Protein Purification and Sample Prep Using PhyTip Columns for Therapeutic Leads Screening and Process Development
Lee Hoang, PhD., PhyNexus, Inc., The 5th Annual Proteins Congress 2012, London
Optimization of Protein Purification Using Small-Scale Separation Columns
Chris Suh, PhD., PhyNexus, Inc., Protein Therapeutics Discovery and Development
PhyNexus Users Group Symposium 2014
Douglas Gjerde, PhyNexus
Lee Hoang, PhyNexus
Select your resin, robotic system, tip and resin volume and see the results in the table below.
C18 Columns
Resin Robotic System Tip Volume Resin Volume QTY/Box Part Number Description
C18 300 Å
Agilent Velocity 11 Bravo & V-Prep
200
20
96
PTV-92-20-19
Box of 96 PhyTip® columns (200 µl volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
Analytik Jena CyBio FeliX
1000
10
96
PTT-91-10-19
Box of 96 PhyTip® columns (1 ml volume) containing 10 µl of C18 (300 Å pore) resin per column
C18 300 Å
Beckman Biomek iSeries
1000
10
96
PTB-91-10-19
Box of 96 PhyTip® columns (1 ml volume) containing 10 µl of C18 (300 Å pore) resin per column
C18 300 Å
Beckman Biomek iSeries
1000
10
96
PTB-91-16-19
Box of 96 PhyTip® columns (1 ml volume) containing 160 µl of C18 (300 Å pore) resin per column
C18 300 Å
Beckman Biomek iSeries
1000
20
96
PTB-91-20-19
Box of 96 PhyTip® columns (1 ml volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
Beckman Biomek iSeries
1000
40
96
PTB-91-40-19
Box of 96 PhyTip® columns (1 ml volume) containing 40 µl of C18 (300 Å pore) resin per column
C18 300 Å
Beckman Biomek iSeries
1000
320
96
PTB-91-80-19
Box of 96 PhyTip® columns (1 ml volume) containing 80 µl of C18 (300 Å pore) resin per column
C18 300 Å
Beckman FX & NX 96-well
200
20
96
PTB-92-20-19
Box of 96 PhyTip® columns (200 µl volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
Beckman FX & NX 96-well
200
5
96
PTB-92-05-19
Box of 96 PhyTip® columns (200 µl volume) containing 5 µl of C18 (300 Å pore) resin per column
C18 300 Å
Caliper SciClone & Zephyr 96 channel_now Perkin Elmer
200
20
96
PTT-92-20-19
Box of 96 PhyTip® columns (200 µl volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
Caliper SciClone & Zephyr 96 channel_now Perkin Elmer
200
5
96
PTT-92-05-19
Box of 96 PhyTip® columns (200 µl volume) containing 5 µl of C18 (300 Å pore) resin per column
C18 300 Å
Dynamic Devices Lynx
1000
10
96
PTX-91-10-19
Box of 96 PhyTip® columns (1 ml volume) containing 10 µl of C18 (300 Å pore) resin per column
C18 300 Å
Dynamic Devices Lynx
1000
10
96
PTX-91-16-19
Box of 96 PhyTip® columns (1 ml volume) containing 160 µl of C18 (300 Å pore) resin per column
C18 300 Å
Dynamic Devices Lynx
1000
20
96
PTX-91-20-19
Box of 96 PhyTip® columns (1 ml volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
Dynamic Devices Lynx
1000
40
96
PTX-91-40-19
Box of 96 PhyTip® columns (1 ml volume) containing 40 µl of C18 (300 Å pore) resin per column
C18 300 Å
Dynamic Devices Lynx
1000
320
96
PTX-91-80-19
Box of 96 PhyTip® columns (1 ml volume) containing 80 µl of C18 (300 Å pore) resin per column
C18 300 Å
Dynamic Devices Lynx
300
20
96
PTX-93-20-19
Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
Dynamic Devices Lynx
300
5
96
PTX-93-05-19
Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of C18 (300 Å pore) resin per column
C18 300 Å
Dynamic Devices Oasis
1000
10
96
PTT-91-10-19
Box of 96 PhyTip® columns (1 ml volume) containing 10 µl of C18 (300 Å pore) resin per column
C18 300 Å
Dynamic Devices Oasis
1000
10
96
PTT-91-16-19
Box of 96 PhyTip® columns (1 ml volume) containing 160 µl of C18 (300 Å pore) resin per column
C18 300 Å
Dynamic Devices Oasis
1000
20
96
PTT-91-20-19
Box of 96 PhyTip® columns (1 ml volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
Dynamic Devices Oasis
1000
40
96
PTT-91-40-19
Box of 96 PhyTip® columns (1 ml volume) containing 40 µl of C18 (300 Å pore) resin per column
C18 300 Å
Dynamic Devices Oasis
1000
320
96
PTT-91-80-19
Box of 96 PhyTip® columns (1 ml volume) containing 80 µl of C18 (300 Å pore) resin per column
C18 300 Å
Dynamic Devices Oasis
200
20
96
PTT-92-20-19
Box of 96 PhyTip® columns (200 µl volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
Dynamic Devices Oasis
200
5
96
PTT-92-05-19
Box of 96 PhyTip® columns (200 µl volume) containing 5 µl of C18 (300 Å pore) resin per column
C18 300 Å
Hamilton Nimbus|STAR|Vantage
1000
10
96
PTH-91-10-19
Box of 96 PhyTip® columns (1 ml volume) containing 10 µl of C18 (300 Å pore) resin per column
C18 300 Å
Hamilton Nimbus|STAR|Vantage
1000
10
96
PTH-91-16-19
Box of 96 PhyTip® columns (1 ml volume) containing 160 µl of C18 (300 Å pore) resin per column
C18 300 Å
Hamilton Nimbus|STAR|Vantage
1000
20
96
PTH-91-20-19
Box of 96 PhyTip® columns (1 ml volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
Hamilton Nimbus|STAR|Vantage
1000
40
96
PTH-91-40-19
Box of 96 PhyTip® columns (1 ml volume) containing 40 µl of C18 (300 Å pore) resin per column
C18 300 Å
Hamilton Nimbus|STAR|Vantage
1000
320
96
PTH-91-80-19
Box of 96 PhyTip® columns (1 ml volume) containing 80 µl of C18 (300 Å pore) resin per column
C18 300 Å
Hamilton Nimbus|STAR|Vantage
300
20
96
PTH-93-20-19
Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
Hamilton Nimbus|STAR|Vantage
300
5
96
PTH-93-05-19
Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of C18 (300 Å pore) resin per column
C18 300 Å
Janus MDT 96-channel
200
20
96
PTP-92-20-19
Box of 96 PhyTip® columns (200 µl volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
Janus MDT 96-channel
200
5
96
PTP-92-05-19
Box of 96 PhyTip® columns (200 µl volume) containing 5 µl of C18 (300 Å pore) resin per column
C18 300 Å
Janus Varispan 4 or 6 wells
1000
10
96
PTT-91-10-19
Box of 96 PhyTip® columns (1 ml volume) containing 10 µl of C18 (300 Å pore) resin per column
C18 300 Å
Janus Varispan 4 or 6 wells
1000
10
96
PTT-91-16-19
Box of 96 PhyTip® columns (1 ml volume) containing 160 µl of C18 (300 Å pore) resin per column
C18 300 Å
Janus Varispan 4 or 6 wells
1000
20
96
PTT-91-20-19
Box of 96 PhyTip® columns (1 ml volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
Janus Varispan 4 or 6 wells
1000
40
96
PTT-91-40-19
Box of 96 PhyTip® columns (1 ml volume) containing 40 µl of C18 (300 Å pore) resin per column
C18 300 Å
Janus Varispan 4 or 6 wells
1000
320
96
PTT-91-80-19
Box of 96 PhyTip® columns (1 ml volume) containing 80 µl of C18 (300 Å pore) resin per column
C18 300 Å
ME and MEA2 - 1200 ul head
1000
10
96
PTR-91-10-19
Box of 96 PhyTip® columns (1 ml volume) containing 10 µl of C18 (300 Å pore) resin per column
C18 300 Å
ME and MEA2 - 1200 ul head
1000
10
96
PTR-91-16-19
Box of 96 PhyTip® columns (1 ml volume) containing 160 µl of C18 (300 Å pore) resin per column
C18 300 Å
ME and MEA2 - 1200 ul head
1000
20
96
PTR-91-20-19
Box of 96 PhyTip® columns (1 ml volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
ME and MEA2 - 1200 ul head
1000
20
96
PTR-91-32-19
Box of 96 PhyTip® columns (1 ml volume) containing 320 µl of C18 (300 Å pore) resin per column
C18 300 Å
ME and MEA2 - 1200 ul head
1000
40
96
PTR-91-40-19
Box of 96 PhyTip® columns (1 ml volume) containing 40 µl of C18 (300 Å pore) resin per column
C18 300 Å
ME and MEA2 - 1200 ul head
1000
320
96
PTR-91-80-19
Box of 96 PhyTip® columns (1 ml volume) containing 80 µl of C18 (300 Å pore) resin per column
C18 300 Å
ME and MEA2 - 200 ul head
200
20
96
PTR-92-20-19
Box of 96 PhyTip® columns (200 µl volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
ME and MEA2 - 200 ul head
200
5
96
PTR-92-05-19
Box of 96 PhyTip® columns (200 µl volume) containing 5 µl of C18 (300 Å pore) resin per column
C18 300 Å
Opentrons OT-2
300
20
96
PTX-93-20-19
Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
Opentrons OT-2
300
5
96
PTX-93-05-19
Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of C18 (300 Å pore) resin per column
C18 300 Å
Perkin Elmer MiniTrack
200
20
96
PTP-92-20-19
Box of 96 PhyTip® columns (200 µl volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
Perkin Elmer MiniTrack
200
5
96
PTP-92-05-19
Box of 96 PhyTip® columns (200 µl volume) containing 5 µl of C18 (300 Å pore) resin per column
C18 300 Å
Tecan Freedom Evo LiHa
1000
10
96
PTT-91-10-19
Box of 96 PhyTip® columns (1 ml volume) containing 10 µl of C18 (300 Å pore) resin per column
C18 300 Å
Tecan Freedom Evo LiHa
1000
10
96
PTT-91-16-19
Box of 96 PhyTip® columns (1 ml volume) containing 160 µl of C18 (300 Å pore) resin per column
C18 300 Å
Tecan Freedom Evo LiHa
1000
20
96
PTT-91-20-19
Box of 96 PhyTip® columns (1 ml volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
Tecan Freedom Evo LiHa
1000
40
96
PTT-91-40-19
Box of 96 PhyTip® columns (1 ml volume) containing 40 µl of C18 (300 Å pore) resin per column
C18 300 Å
Tecan Freedom Evo LiHa
1000
320
96
PTT-91-80-19
Box of 96 PhyTip® columns (1 ml volume) containing 80 µl of C18 (300 Å pore) resin per column
C18 300 Å
Tecan Freedom Evo LiHa
200
20
96
PTT-92-20-19
Box of 96 PhyTip® columns (200 µl volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
Tecan Freedom Evo LiHa
200
5
96
PTT-92-05-19
Box of 96 PhyTip® columns (200 µl volume) containing 5 µl of C18 (300 Å pore) resin per column
C18 300 Å
Tecan Freedom Evo MCA
200
20
96
PTT-92-20-19
Box of 96 PhyTip® columns (200 µl volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
Tecan Freedom Evo MCA
200
5
96
PTT-92-05-19
Box of 96 PhyTip® columns (200 µl volume) containing 5 µl of C18 (300 Å pore) resin per column
C18 300 Å
Tecan Freedom Evo MCA
500
10
96
PTT-95-16-19
Box of 96 PhyTip® columns (500 µl volume) containing 160 µl of C18 (300 Å pore) resin per column
C18 300 Å
Tecan Freedom Evo MCA
500
20
96
PTT-95-20-19
Box of 96 PhyTip® columns (500 µl volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
Tecan Freedom Evo MCA
500
40
96
PTT-95-40-19
Box of 96 PhyTip® columns (500 µl volume) containing 40 µl of C18 (300 Å pore) resin per column
C18 300 Å
Tecan Freedom Evo MCA
500
320
96
PTT-95-80-19
Box of 96 PhyTip® columns (500 µl volume) containing 80 µl of C18 (300 Å pore) resin per column
C18 300 Å
Tecan Te-Mo
200
20
96
PTT-92-20-19
Box of 96 PhyTip® columns (200 µl volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
Tecan Te-Mo
200
5
96
PTT-92-05-19
Box of 96 PhyTip® columns (200 µl volume) containing 5 µl of C18 (300 Å pore) resin per column
C18 300 Å
Analytik Jena CyBio FeliX
1000
160
96
PTT-91-16-19
Box of 96 PhyTip® columns (1 ml volume) containing 160 µl of C18 (300 Å pore) resin per column
C18 300 Å
Analytik Jena CyBio FeliX
1000
20
96
PTT-91-20-19
Box of 96 PhyTip® columns (1 ml volume) containing 20 µl of C18 (300 Å pore) resin per column
C18 300 Å
Analytik Jena CyBio FeliX
1000
40
96
PTT-91-40-19
Box of 96 PhyTip® columns (1 ml volume) containing 40 µl of C18 (300 Å pore) resin per column
C18 300 Å
Analytik Jena CyBio FeliX
1000
80
96
PTT-91-80-19
Box of 96 PhyTip® columns (1 ml volume) containing 80 µl of C18 (300 Å pore) resin per column
C18 90 Å
Analytik Jena CyBio FeliX
1000
10
96
PTT-91-10-18
Box of 96 PhyTip® columns (1 ml volume) containing 10 µl of C18 (90Å pore) resin per column
C18 90 Å
Agilent Velocity 11 Bravo & V-Prep
200
20
96
PTV-92-20-18
Box of 96 PhyTip® columns (200 µl volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
Beckman Biomek iSeries
1000
10
96
PTB-91-10-18
Box of 96 PhyTip® columns (1 ml volume) containing 10 µl of C18 (90Å pore) resin per column
C18 90 Å
Beckman Biomek iSeries
1000
10
96
PTB-91-16-18
Box of 96 PhyTip® columns (1 ml volume) containing 160 µl of C18 (90Å pore) resin per column
C18 90 Å
Beckman Biomek iSeries
1000
20
96
PTB-91-20-18
Box of 96 PhyTip® columns (1 ml volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
Beckman Biomek iSeries
1000
40
96
PTB-91-40-18
Box of 96 PhyTip® columns (1 ml volume) containing 40 µl of C18 (90Å pore) resin per column
C18 90 Å
Beckman Biomek iSeries
1000
320
96
PTB-91-80-18
Box of 96 PhyTip® columns (1 ml volume) containing 80 µl of C18 (90Å pore) resin per column
C18 90 Å
Beckman FX & NX 96-well
200
20
96
PTB-92-20-18
Box of 96 PhyTip® columns (200 µl volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
Beckman FX & NX 96-well
200
5
96
PTB-92-05-18
Box of 96 PhyTip® columns (200 µl volume) containing 5 µl of C18 (90Å pore) resin per column
C18 90 Å
Caliper SciClone & Zephyr 96 channel_now Perkin Elmer
200
20
96
PTT-92-20-18
Box of 96 PhyTip® columns (200 µl volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
Caliper SciClone & Zephyr 96 channel_now Perkin Elmer
200
5
96
PTT-92-05-18
Box of 96 PhyTip® columns (200 µl volume) containing 5 µl of C18 (90Å pore) resin per column
C18 90 Å
Dynamic Devices Lynx
1000
10
96
PTX-91-10-18
Box of 96 PhyTip® columns (1 ml volume) containing 10 µl of C18 (90Å pore) resin per column
C18 90 Å
Dynamic Devices Lynx
1000
10
96
PTX-91-16-18
Box of 96 PhyTip® columns (1 ml volume) containing 160 µl of C18 (90Å pore) resin per column
C18 90 Å
Dynamic Devices Lynx
1000
20
96
PTX-91-20-18
Box of 96 PhyTip® columns (1 ml volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
Dynamic Devices Lynx
1000
40
96
PTX-91-40-18
Box of 96 PhyTip® columns (1 ml volume) containing 40 µl of C18 (90Å pore) resin per column
C18 90 Å
Dynamic Devices Lynx
1000
320
96
PTX-91-80-18
Box of 96 PhyTip® columns (1 ml volume) containing 80 µl of C18 (90Å pore) resin per column
C18 90 Å
Dynamic Devices Lynx
300
20
96
PTX-93-20-18
Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
Dynamic Devices Lynx
300
5
96
PTX-93-05-18
Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of C18 (90Å pore) resin per column
C18 90 Å
Dynamic Devices Oasis
1000
10
96
PTT-91-10-18
Box of 96 PhyTip® columns (1 ml volume) containing 10 µl of C18 (90Å pore) resin per column
C18 90 Å
Dynamic Devices Oasis
1000
10
96
PTT-91-16-18
Box of 96 PhyTip® columns (1 ml volume) containing 160 µl of C18 (90Å pore) resin per column
C18 90 Å
Dynamic Devices Oasis
1000
20
96
PTT-91-20-18
Box of 96 PhyTip® columns (1 ml volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
Dynamic Devices Oasis
1000
40
96
PTT-91-40-18
Box of 96 PhyTip® columns (1 ml volume) containing 40 µl of C18 (90Å pore) resin per column
C18 90 Å
Dynamic Devices Oasis
1000
320
96
PTT-91-80-18
Box of 96 PhyTip® columns (1 ml volume) containing 80 µl of C18 (90Å pore) resin per column
C18 90 Å
Dynamic Devices Oasis
200
20
96
PTT-92-20-18
Box of 96 PhyTip® columns (200 µl volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
Dynamic Devices Oasis
200
5
96
PTT-92-05-18
Box of 96 PhyTip® columns (200 µl volume) containing 5 µl of C18 (90Å pore) resin per column
C18 90 Å
Hamilton Nimbus|STAR|Vantage
1000
10
96
PTH-91-10-18
Box of 96 PhyTip® columns (1 ml volume) containing 10 µl of C18 (90Å pore) resin per column
C18 90 Å
Hamilton Nimbus|STAR|Vantage
1000
10
96
PTH-91-16-18
Box of 96 PhyTip® columns (1 ml volume) containing 160 µl of C18 (90Å pore) resin per column
C18 90 Å
Hamilton Nimbus|STAR|Vantage
1000
20
96
PTH-91-20-18
Box of 96 PhyTip® columns (1 ml volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
Hamilton Nimbus|STAR|Vantage
1000
40
96
PTH-91-40-18
Box of 96 PhyTip® columns (1 ml volume) containing 40 µl of C18 (90Å pore) resin per column
C18 90 Å
Hamilton Nimbus|STAR|Vantage
1000
320
96
PTH-91-80-18
Box of 96 PhyTip® columns (1 ml volume) containing 80 µl of C18 (90Å pore) resin per column
C18 90 Å
Hamilton Nimbus|STAR|Vantage
300
20
96
PTH-93-20-18
Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
Hamilton Nimbus|STAR|Vantage
300
5
96
PTH-93-05-18
Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of C18 (90Å pore) resin per column
C18 90 Å
Janus MDT 96-channel
200
20
96
PTP-92-20-18
Box of 96 PhyTip® columns (200 µl volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
Janus MDT 96-channel
200
5
96
PTP-92-05-18
Box of 96 PhyTip® columns (200 µl volume) containing 5 µl of C18 (90Å pore) resin per column
C18 90 Å
Janus Varispan 4 or 6 wells
1000
10
96
PTT-91-10-18
Box of 96 PhyTip® columns (1 ml volume) containing 10 µl of C18 (90Å pore) resin per column
C18 90 Å
Janus Varispan 4 or 6 wells
1000
10
96
PTT-91-16-18
Box of 96 PhyTip® columns (1 ml volume) containing 160 µl of C18 (90Å pore) resin per column
C18 90 Å
Janus Varispan 4 or 6 wells
1000
20
96
PTT-91-20-18
Box of 96 PhyTip® columns (1 ml volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
Janus Varispan 4 or 6 wells
1000
40
96
PTT-91-40-18
Box of 96 PhyTip® columns (1 ml volume) containing 40 µl of C18 (90Å pore) resin per column
C18 90 Å
Janus Varispan 4 or 6 wells
1000
320
96
PTT-91-80-18
Box of 96 PhyTip® columns (1 ml volume) containing 80 µl of C18 (90Å pore) resin per column
C18 90 Å
ME and MEA2 - 1200 ul head
1000
10
96
PTR-91-10-18
Box of 96 PhyTip® columns (1 ml volume) containing 10 µl of C18 (90Å pore) resin per column
C18 90 Å
ME and MEA2 - 1200 ul head
1000
10
96
PTR-91-16-18
Box of 96 PhyTip® columns (1 ml volume) containing 160 µl of C18 (90Å pore) resin per column
C18 90 Å
ME and MEA2 - 1200 ul head
1000
20
96
PTR-91-20-18
Box of 96 PhyTip® columns (1 ml volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
ME and MEA2 - 1200 ul head
1000
20
96
PTR-91-32-18
Box of 96 PhyTip® columns (1 ml volume) containing 320 µl of C18 (90Å pore) resin per column
C18 90 Å
ME and MEA2 - 1200 ul head
1000
40
96
PTR-91-40-18
Box of 96 PhyTip® columns (1 ml volume) containing 40 µl of C18 (90Å pore) resin per column
C18 90 Å
ME and MEA2 - 1200 ul head
1000
320
96
PTR-91-80-18
Box of 96 PhyTip® columns (1 ml volume) containing 80 µl of C18 (90Å pore) resin per column
C18 90 Å
ME and MEA2 - 200 ul head
200
20
96
PTR-92-20-18
Box of 96 PhyTip® columns (200 µl volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
ME and MEA2 - 200 ul head
200
5
96
PTR-92-05-18
Box of 96 PhyTip® columns (200 µl volume) containing 5 µl of C18 (90Å pore) resin per column
C18 90 Å
Opentrons OT-2
300
20
96
PTX-93-20-18
Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
Opentrons OT-2
300
5
96
PTX-93-05-18
Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of C18 (90Å pore) resin per column
C18 90 Å
Perkin Elmer MiniTrack
200
20
96
PTP-92-20-18
Box of 96 PhyTip® columns (200 µl volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
Perkin Elmer MiniTrack
200
5
96
PTP-92-05-18
Box of 96 PhyTip® columns (200 µl volume) containing 5 µl of C18 (90Å pore) resin per column
C18 90 Å
Tecan Freedom Evo LiHa
1000
10
96
PTT-91-10-18
Box of 96 PhyTip® columns (1 ml volume) containing 10 µl of C18 (90Å pore) resin per column
C18 90 Å
Tecan Freedom Evo LiHa
1000
10
96
PTT-91-16-18
Box of 96 PhyTip® columns (1 ml volume) containing 160 µl of C18 (90Å pore) resin per column
C18 90 Å
Tecan Freedom Evo LiHa
1000
20
96
PTT-91-20-18
Box of 96 PhyTip® columns (1 ml volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
Tecan Freedom Evo LiHa
1000
40
96
PTT-91-40-18
Box of 96 PhyTip® columns (1 ml volume) containing 40 µl of C18 (90Å pore) resin per column
C18 90 Å
Tecan Freedom Evo LiHa
1000
320
96
PTT-91-80-18
Box of 96 PhyTip® columns (1 ml volume) containing 80 µl of C18 (90Å pore) resin per column
C18 90 Å
Tecan Freedom Evo LiHa
200
20
96
PTT-92-20-18
Box of 96 PhyTip® columns (200 µl volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
Tecan Freedom Evo LiHa
200
5
96
PTT-92-05-18
Box of 96 PhyTip® columns (200 µl volume) containing 5 µl of C18 (90Å pore) resin per column
C18 90 Å
Tecan Freedom Evo MCA
200
20
96
PTT-92-20-18
Box of 96 PhyTip® columns (200 µl volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
Tecan Freedom Evo MCA
200
5
96
PTT-92-05-18
Box of 96 PhyTip® columns (200 µl volume) containing 5 µl of C18 (90Å pore) resin per column
C18 90 Å
Tecan Freedom Evo MCA
500
10
96
PTT-95-16-18
Box of 96 PhyTip® columns (500 µl volume) containing 160 µl of C18 (90Å pore) resin per column
C18 90 Å
Tecan Freedom Evo MCA
500
20
96
PTT-95-20-18
Box of 96 PhyTip® columns (500 µl volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
Tecan Freedom Evo MCA
500
40
96
PTT-95-40-18
Box of 96 PhyTip® columns (500 µl volume) containing 40 µl of C18 (90Å pore) resin per column
C18 90 Å
Tecan Freedom Evo MCA
500
320
96
PTT-95-80-18
Box of 96 PhyTip® columns (500 µl volume) containing 80 µl of C18 (90Å pore) resin per column
C18 90 Å
Tecan Te-Mo
200
20
96
PTT-92-20-18
Box of 96 PhyTip® columns (200 µl volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
Tecan Te-Mo
200
5
96
PTT-92-05-18
Box of 96 PhyTip® columns (200 µl volume) containing 5 µl of C18 (90Å pore) resin per column
C18 90 Å
Analytik Jena CyBio FeliX
1000
160
96
PTT-91-16-18
Box of 96 PhyTip® columns (1 ml volume) containing 160 µl of C18 (90Å pore) resin per column
C18 90 Å
Analytik Jena CyBio FeliX
1000
20
96
PTT-91-20-18
Box of 96 PhyTip® columns (1 ml volume) containing 20 µl of C18 (90Å pore) resin per column
C18 90 Å
Analytik Jena CyBio FeliX
1000
40
96
PTT-91-40-18
Box of 96 PhyTip® columns (1 ml volume) containing 40 µl of C18 (90Å pore) resin per column
C18 90 Å
Analytik Jena CyBio FeliX
1000
80
96
PTT-91-80-18
Box of 96 PhyTip® columns (1 ml volume) containing 80 µl of C18 (90Å pore) resin per column