Through the use of sharp isotherm chemistry, the PhyNexus Dual Flow technology may be employed with most types of chromatography resins. These include normal phase PhyTip columns, which can capture polar molecules. Reverse phase PhyTip columns may be used to capture ionic materials, when used with an ion-pairing reagent. They may be also used to capture non-polar or slightly polar molecules directly. Anion and cations, such as proteins, salts, nucleic acids, etc. may be captured with anion exchangers and cation exchangers, depending on the charge and isolelectric point of the target molecule.