The ProPlus and ProPlus LX resin ligand is a recombinant Protein A derived ligand that demonstrates attributes of both Protein A and Protein G. ProPlus and ProPlus LX share a number of features including a modified Protein A ligand designed for alkali stability. Also, the modified Protein A is coupled to the resin matrix in an orientation that maximizes capacity. Choose ProPlus LX for high titer cultures to take advantage of high dynamic binding capacity. In certain cases, Protein G affinity resin will still demonstrate  superior selectivity over Protein A such as for certain isotypes and species of IgGs, including some mouse and rat IgGs.

ProPlus and ProPlus LX

  • Modified Protein A increases selectivity for Mouse IgGs
  • Optimal capacity due to ligand orientation
  • Capture, purify and enrich in as little as 15 minutes to obtain high concentrations of fully functional protein
  • Process small sample volumes in a reproducible, high throughput, automated format
  • Elution volumes as low as 10 μL, producing enrichment factors as high as 50 fold,  with concentrations of purified protein of up to 10 mg/mL
  • At least 10% higher capacity than Protein A resins
  • Broad range of selectivity, recognizing various isotypes and species, similar to Protein G
  • 3-5 fold greater recovery than Protein G

ProPlus LX

  • High dynamic binding capacity for high titer cultures
Description

ProPlus / ProPlus LX

The resin ligand for ProPlus and ProPlus LX is a Protein A derived recombinant ligand that demonstrates selectivity attributes of both Protein A and Protein G. Protein G demonstrates superior selectivity over Protein A for certain isotypes and species of IgGs, such as mouse and rat IgGs. However, Protein G has lower capacity and therefore is not as desirable when higher recovery is required. The PhyTip ProPlus and ProPlus LX columns demonstrate similar selectivity to Protein G and higher capacity than Protein A allowing for the best of both worlds. PhyTip ProPlus and ProPlus LX columns are ideal for antibody screening from hybridoma supernatants or for high throughput extraction and purification of expressed antibodies. IgG binds ProPlus with high affinity and specificity. The strength and selectivity of this interaction enables ProPlus to effectively purify IgGs from complex protein mixtures.

  • ProPlus / ProPlus LX columns are selective for various species and isotypes of IgG, similar to Protein G, and can purify mouse IgGs from hybridomas.
  • ProPlus / ProPlus LX demonstrate high capacity for bound IgGs.
  • The resin bed volume of PhyTip ProPlus / ProPlus LX columns can be adjusted to recover the maximum amount of antibody.
  • ProPlus LX provides high dynamic binding capacity for high titer cultures
Application

PhyTip columns are unique capture, purification and enrichmentTM tools from PhyNexus designed for micro volume protein sample preparation. PhyTip Columns are available for a variety of liquid handling platforms and contain specific affinity resins for application specific requirements.

Data

PhyNexus recommends the use of the PhyTip Starter Kit containing IgG as a standard to verify the use of PhyTip columns with all applications.

Samples for purification and enrichment must be clear and free from particulate matter. It is highly recommended to centrifuge samples and use the clear supernatant only, prior to use with PhyTip columns.

Shipping and Storage

Each pack of PhyTip columns has been manufactured and QC’d to the highest standards and shipped in retainer boxes that maintain the integrity of the specific affinity resin within each PhyTip column. This product is shipped at ambient temperatures, but on receipt should be stored in a standard laboratory refrigerator between 4 and 8◦C.

  • Do NOT freeze or store frozen.
  • When not in use, keep the lid of the box closed and sealed, store in the refrigerator.
  • Do not allow affinity resin to dry out by extended storage in a dry environment.

PhyTip columns with ProPlus / ProPlus LX are stored in Glycerol when shipped from PhyNexus.

Documents
Robotic Systems Compatibility

ProPlus / ProPlus LX PhyTip Columns are available for these robotic systems.



Part Numbers

ProPlus / ProPlus LX PhyTip Columns