The ProPlus and ProPlus LX resin ligand is a recombinant Protein A derived ligand that demonstrates attributes of both Protein A and Protein G. ProPlus and ProPlus LX share a number of features including a modified Protein A ligand designed for alkali stability. Also, the modified Protein A is coupled to the resin matrix in an orientation that maximizes capacity. Choose ProPlus LX for high titer cultures to take advantage of high dynamic binding capacity. In certain cases, Protein G affinity resin will still demonstrate  superior selectivity over Protein A such as for certain isotypes and species of IgGs, including some mouse and rat IgGs.

ProPlus and ProPlus LX

  • Modified Protein A increases selectivity for Mouse IgGs
  • Optimal capacity due to ligand orientation
  • Capture, purify and enrich in as little as 15 minutes to obtain high concentrations of fully functional protein
  • Process small sample volumes in a reproducible, high throughput, automated format
  • Elution volumes as low as 10 μL, producing enrichment factors as high as 50 fold,  with concentrations of purified protein of up to 10 mg/mL
  • At least 10% higher capacity than Protein A resins
  • Broad range of selectivity, recognizing various isotypes and species, similar to Protein G
  • 3-5 fold greater recovery than Protein G

ProPlus LX

  • High dynamic binding capacity for high titer cultures

ProPlus / ProPlus LX columns with our proprietary Dual Flow Chromatography provide complete antibody binding and high final concentrations. PhyTip columns are compatible with most 8, 12, and 96-channel automation liquid handling robots including Agilent TechnologiesBeckman CoulterDynamic DevicesPerkin ElmerHamilton, and Tecan.

Legal Information
MabSelect SuRe and MabSelect SuRe LX are trademarks of GE Healthcare.

PhyNexus ProPlus and ProPlus LX MabSelect Sure

ProPlus/ProPlus LX tips (MabSelect SuRe™) are available in tip volumes (µL) of 200|300|500|1000 and resin volumes (µL) of 5|10|20|40|80|160|320 depending upon robot configuration.

Description

ProPlus/ProPlus LX (MabSelect SuRe™)

The resin ligand for ProPlus and ProPlus LX is a Protein A derived recombinant ligand that demonstrates selectivity attributes of both Protein A and Protein G. Protein G demonstrates superior selectivity over Protein A for certain isotypes and species of IgGs, such as mouse and rat IgGs. However, Protein G has lower capacity and therefore is not as desirable when higher recovery is required. The PhyTip ProPlus and ProPlus LX columns demonstrate similar selectivity to Protein G and higher capacity than Protein A allowing for the best of both worlds. PhyTip ProPlus and ProPlus LX columns are ideal for antibody screening from hybridoma supernatants or for high throughput extraction and purification of expressed antibodies. IgG binds ProPlus with high affinity and specificity. The strength and selectivity of this interaction enables ProPlus to effectively purify IgGs from complex protein mixtures.

  • ProPlus/ProPlus LX columns are selective for various species and isotypes of IgG, similar to Protein G, and can purify mouse IgGs from hybridomas.
  • ProPlus/ProPlus LX demonstrate high capacity for bound IgGs.
  • The resin bed volume of PhyTip ProPlus/ProPlus LX columns can be adjusted to recover the maximum amount of antibody.
  • ProPlus LX provides high dynamic binding capacity for high titer cultures
Application

PhyTip columns are unique capture, purification and enrichment tools from PhyNexus designed for micro volume protein sample preparation. PhyTip Columns are available for a variety of liquid handling platforms and contain specific affinity resins for application specific requirements.

Data

PhyNexus recommends the use of the PhyTip Starter Kit containing IgG as a standard to verify the use of PhyTip columns with all applications.

Samples for purification and enrichment must be clear and free from particulate matter. It is highly recommended to centrifuge samples and use the clear supernatant only, prior to use with PhyTip columns.

Shipping and Storage

Each pack of PhyTip columns has been manufactured and QC’d to the highest standards and shipped in retainer boxes that maintain the integrity of the specific affinity resin within each PhyTip column. This product is shipped at ambient temperatures, but on receipt should be stored in a standard laboratory refrigerator between 4 and 8◦C.

  • Do NOT freeze or store frozen.
  • When not in use, keep the lid of the box closed and sealed, store in the refrigerator.
  • Do not allow affinity resin to dry out by extended storage in a dry environment.

PhyTip columns with ProPlus / ProPlus LX are stored in Glycerol when shipped from PhyNexus.

Documents
Compatibility

ProPlus / ProPlus LX PhyTip Columns are available for these robotic systems.

Citations

Posters


Screening for Chromatographic Success and Failure on Small Scale
W. Gillette (NCI/SAIC-Frederick)

Space is the Place: Using DOE and Microscale Techniques to Define Process Boundaries
M. Wenger (Merck & Co.)

Development of Small-Scale, High Throughput, Chromatographic Protein Purification
K. Valente1, M. Wenger2, J. Shanter1, S. Sagar1, J. Konz1 (1Merck Biopurification Development, 2Merck Bioprocess and Bioanalytical Research)

Automated sample preparation facilitated by PhyNexus MEA Purification System for oligosaccharide mapping of therapeutic glycoproteins
B. Prater, K. Anumula, J. Patti, J. Hutchins (Inhibitex)

Accelerated antigen identification: a case study
Raven biotechnologies, inc.

A flexible platform for automated high-performance protein purification using micro scale separation technology
J. Lambert, M. Anderson, D. Gjerde, L. Nguyen (PhyNexus)

Micro-scale protein enrichment using novel affinity column technology
J. Lambert, U. Banik, C. Hanna (PhyNexus)

A novel approach to automated high-throughput protein enrichment and characterization
J. Lambert (PhyNexus); L. Jordan, A. Esterman, S. Cohen (Caliper)

A novel approach to high-throughput monoclonal and recombinant antibody enrichment and characterization
J. Lambert, C. Hanna, U. Banik (PhyNexus); D. Sexton, K. Kopacz (Dyax); S. Wiltshire (HTS Biosystems)

High-throughput sample processing and affinity characterization of antibodies and recombinant proteins
S. Wiltshire, J. Lambert (HTS Biosystems); C. Hanna (PhyNexus)

Innovative Solutions for Automated Protein and Nucleic Acid Purification
Lee Hoang, PhyNexus, Inc., PhyNexus Webinar

Optimizing Efficiency and Effectiveness When Expressing Proteins for Drug Discovery
Krista Bowman, Genentech, Inc., Protein Engineering Summit (PEGS) 2012

Automated, High Throughput Protein Purification and Sample Prep Using PhyTip Columns for Therapeutic Leads Screening and Process Development
Lee Hoang, PhD., PhyNexus, Inc., The 5th Annual Proteins Congress 2012, London

Identification of Unknown Target Antigens Using PhyNexus Technology
Claudia Fieger, Tim Hotaling, MarcroGenics, Inc.

Optimization of Protein Purification Using Small-Scale Separation Columns
Chris Suh, PhD., PhyNexus, Inc., Protein Therapeutics Discovery and Development

PhyNexus Users Group Symposium 2014
Douglas Gjerde, PhyNexus
Lee Hoang, PhyNexus
Alice Yam, Sutro

Select your resin, robotic system, tip and resin volume and see the results in the table below.

ProPlus/ProPlus LX (MabSelect SuRe™) PhyTip Columns