Opentrons OT-2
The OT-2 from Opentrons is an affordable, easy-to-use pipetting robot that uses integrated electronic pipettes to transfer liquids and run experiments for biologists in their labs. These electronic pipettes are a novel design created by Opentrons, and provide scientists with fast, accurate, and precise liquid transfers.
Biotage PhyTip columns have been designed and validated for the Opentrons OT-2 robot equipped with the 8-channel 300 µL Opentrons pipette.
Biotage PhyTip columns used with our proprietary Dual Flow Chromatography provide complete antibody binding for highly concentrated sample recovery from small samples.
OT-2 PhyTip Columns protein purification protocol
Select your resin, robotic system, tip and resin volume and see the results in the table below.
PhyTip Columns for opentrons OT-2 Robot
| Resin | Robotic System | Tip Volume | Resin Volume | QTY/Box | Part Number | Description |
|---|---|---|---|---|---|---|
| C18 300 Å | Opentrons OT-2 | 300 | 20 | 96 | PTX-93-20-19 | Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of C18 (300 Å pore) resin per column |
| C18 300 Å | Opentrons OT-2 | 300 | 5 | 96 | PTX-93-05-19 | Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of C18 (300 Å pore) resin per column |
| C18 90 Å | Opentrons OT-2 | 300 | 20 | 96 | PTX-93-20-18 | Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of C18 (90Å pore) resin per column |
| C18 90 Å | Opentrons OT-2 | 300 | 5 | 96 | PTX-93-05-18 | Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of C18 (90Å pore) resin per column |
| C4 300 Å | Opentrons OT-2 | 300 | 20 | 96 | PTX-93-20-52 | Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of C4 (300 Å pore) resin per column |
| C4 300 Å | Opentrons OT-2 | 300 | 5 | 96 | PTX-93-05-52 | Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of C4 (300 Å pore) resin per column |
| CaptureSelect FcXL | Opentrons OT-2 | 300 | 20 | 96 | PTX-93-20-32 | Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of CaptureSelect FcXL resin per column |
| CaptureSelect FcXL | Opentrons OT-2 | 300 | 5 | 96 | PTX-93-05-32 | Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of CaptureSelect FcXL resin per column |
| CaptureSelect IgG-Fc ms | Opentrons OT-2 | 300 | 20 | 96 | PTX-93-20-24 | Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of CaptureSelect IgG-Fc (ms) resin per column |
| CaptureSelect IgG-Fc ms | Opentrons OT-2 | 300 | 5 | 96 | PTX-93-05-24 | Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of CaptureSelect IgG-Fc (ms) resin per column |
| CaptureSelect KappaXL | Opentrons OT-2 | 300 | 20 | 96 | PTX-93-20-31 | Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of CaptureSelect KappaXL resin per column |
| CaptureSelect KappaXL | Opentrons OT-2 | 300 | 5 | 96 | PTX-93-05-31 | Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of CaptureSelect KappaXL resin per column |
| CaptureSelect LC-Lambda Hu | Opentrons OT-2 | 300 | 20 | 96 | PTX-93-20-22 | Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of CaptureSelect LC-lambda(Hu) resin per column |
| CaptureSelect LC-Lambda Hu | Opentrons OT-2 | 300 | 5 | 96 | PTX-93-05-22 | Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of CaptureSelect LC-lambda(Hu) resin per column |
| Glutathione | Opentrons OT-2 | 300 | 20 | 96 | PTX-93-20-04 | Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of Glutathione affinity resin per column |
| Glutathione | Opentrons OT-2 | 300 | 5 | 96 | PTX-93-05-04 | Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of Glutathione affinity resin per column |
| IMAC | Opentrons OT-2 | 300 | 20 | 96 | PTX-93-20-03 | Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of IMAC affinity resin per column |
| IMAC | Opentrons OT-2 | 300 | 5 | 96 | PTX-93-05-03 | Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of IMAC affinity resin per column |
| Normal Phase 1 | Opentrons OT-2 | 300 | 20 | 96 | PTX-93-20-09 | Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of Normal Phase 1 resin per column |
| Normal Phase 1 | Opentrons OT-2 | 300 | 5 | 96 | PTX-93-05-09 | Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of Normal Phase 1 resin per column |
| POROS CaptureSelect IgM | Opentrons OT-2 | 300 | 20 | 96 | PTX-93-20-30 | Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of POROS CaptureSelect IgM resin per column |
| POROS CaptureSelect IgM | Opentrons OT-2 | 300 | 5 | 96 | PTX-93-05-30 | Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of POROS CaptureSelect IgM resin per column |
| ProPlus - MabSelect SuRe™ | Opentrons OT-2 | 300 | 20 | 96 | PTX-93-20-07 | Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of ProPlus (MabSelect SuRe™) affinity resin per column |
| ProPlus - MabSelect SuRe™ | Opentrons OT-2 | 300 | 5 | 96 | PTX-93-05-07 | Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of ProPlus (MabSelect SuRe™) affinity resin per column |
| ProPlus LX - MabSelect SuRe™ LX | Opentrons OT-2 | 300 | 20 | 96 | PTX-93-20-26 | Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of ProPlus LX (MabSelect SuRe™ LX) affinity resin per column |
| ProPlus LX - MabSelect SuRe™ LX | Opentrons OT-2 | 300 | 5 | 96 | PTX-93-05-26 | Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of ProPlus LX (MabSelect SuRe™ LX) affinity resin per column |
| Protein A | Opentrons OT-2 | 300 | 20 | 96 | PTX-93-20-01 | Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of Protein A affinity resin per column |
| Protein A | Opentrons OT-2 | 300 | 5 | 96 | PTX-93-05-01 | Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of Protein A affinity resin per column |
| Protein G | Opentrons OT-2 | 300 | 20 | 96 | PTX-93-20-02 | Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of Protein G affinity resin per column |
| Protein G | Opentrons OT-2 | 300 | 5 | 96 | PTX-93-05-02 | Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of Protein G affinity resin per column |
| Streptavidin | Opentrons OT-2 | 300 | 20 | 96 | PTX-93-20-05 | Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of Streptavidin resin per column |
| Streptavidin | Opentrons OT-2 | 300 | 5 | 96 | PTX-93-05-05 | Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of Streptavidin resin per column |
| Strong Anion | Opentrons OT-2 | 300 | 20 | 96 | PTX-93-20-11 | Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of Strong Anion resin per column |
| Strong Anion | Opentrons OT-2 | 300 | 5 | 96 | PTX-93-05-11 | Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of Strong Anion resin per column |
| Strong Cation | Opentrons OT-2 | 300 | 20 | 96 | PTX-93-20-13 | Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of Strong Cation resin per column |
| Strong Cation | Opentrons OT-2 | 300 | 5 | 96 | PTX-93-05-13 | Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of Strong Cation resin per column |
| Weak Anion | Opentrons OT-2 | 300 | 20 | 96 | PTX-93-20-12 | Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of Weak Anion resin per column |
| Weak Anion | Opentrons OT-2 | 300 | 5 | 96 | PTX-93-05-12 | Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of Weak Anion resin per column |
| Weak Cation | Opentrons OT-2 | 300 | 20 | 96 | PTX-93-20-14 | Box of 96 PhyTip® columns (300 µl volume) containing 20 µl of Weak Cation resin per column |
| Weak Cation | Opentrons OT-2 | 300 | 5 | 96 | PTX-93-05-14 | Box of 96 PhyTip® columns (300 µl volume) containing 5 µl of Weak Cation resin per column |