Say goodbye to problematic plasmid preps with Lysate Direct PhyTip® Columns for high throughput (HTP) Plasmid DNA purification.

At last, a reliable solution for truly walk-away, fully automated HTP plasmid DNA purification.

96 Plasmid Phytip Columns

  • No filtration or centrifugation of lysate required
  • Consistent purification of up to 15 μg
  • Transfection-ready, sequencing-ready
  • 96 samples at a time
  • Suitable for downstream assays
  • Fast and fully automated from the cell pellet
Description

The pace of innovation from life science researchers is constantly pushing the limits of available tools. While technologies such as high throughput construct screening, crystallography and DNA sequencing have advanced, techniques for performing plasmid DNA minipreps have remained fairly unchanged. At the same time greater emphasis is being placed on utilizing automated liquid handling systems to adapt traditional plasmid DNA purification formats like filter plates and magnetic beads to higher throughput, but with limited success. The major challenge to these approaches lies in the complexity of the lysed sample. Current methods require removal of precipitates and debris from bacterial cell lysate prior to DNA binding step by centrifugation or vacuum filtration and ‘careful transfer’. Centrifugation is difficult to automate and requires human intervention. Filtration and transfer methods often result in clogged pipet tips, transfer of precipitates to filters, inconsistent fluid flow, and cross contamination between samples. Such obstacles to high throughput have made currently available methods for automating plasmid DNA minipreps less than satisfactory. PhyNexus has developed a solution for obtaining reproducible automated plasmid DNA minipreps in a truly walk-away fashion. Lysate Direct PhyTip® Column technology consists of a purification column in a pipet tip format. These novel purification columns are composed of pipet tip containing plasmid DNA binding resin and a thin, inert frit screen at the end of the tip to retain the resin. The unique design of the column allows for DNA binding directly from cell lysates in the presence of precipitates and debris without sample clearing. By eliminating the need for centrifugation or filtration, Lysate Direct PhyTip® Columns for Plasmid DNA Purification enable utilization of liquid handing automation found in high throughput laboratories to easily purify up to 96 samples in parallel.

Documents
Robotics Systems Compatiblity

HTP Miniprep PhyTip Columns are available for these robotic systems.



Part Numbers

HTP Miniprep PhyTip columns