Say goodbye to problematic plasmid preps with Lysate Direct PhyTip columns for high throughput HTP plasmid DNA purification.

At last, a reliable solution for truly walk-away, fully automated HTP plasmid DNA purification with your liquid handling robot.
96 Plasmid Phytip Columns

  • No filtration or centrifugation of lysate required
  • Consistent purification of up to 15 μg
  • Transfection grade, sequencing-ready
  • 96 samples at a time
  • Suitable for downstream assays
  • Fast and fully automated from the cell pellet

High throughput Miniprep plasmid columns with our proprietary Dual Flow Chromatography provide complete antibody binding and high final concentrations. PhyTip columns are compatible with most 8, 12, and 96-channel automation liquid handling robots including Beckman Coulter Beckman FX & NX 96-wellDynamic Devices Oasis & LynxHamilton STAR & Nimbus, and TecanFreedom Evo MCA 96-well.

CitationGenentech and PhyNexus Publication – Implementation of High Throughput DNA Production Pipeline

 

Description

The pace of innovation from life science researchers is constantly pushing the limits of available tools. While technologies such as high throughput construct screening, crystallography and DNA sequencing have advanced, techniques for performing plasmid DNA minipreps have remained fairly unchanged. At the same time greater emphasis is being placed on utilizing automated liquid handling systems to adapt traditional plasmid DNA purification formats like filter plates and magnetic beads to higher throughput, but with limited success. The major challenge to these approaches lies in the complexity of the lysed sample. Current methods require removal of precipitates and debris from bacterial cell lysate prior to DNA binding step by centrifugation or vacuum filtration and ‘careful transfer’. Centrifugation is difficult to automate and requires human intervention. Filtration and transfer methods often result in clogged pipet tips, transfer of precipitates to filters, inconsistent fluid flow, and cross contamination between samples. Such obstacles to high throughput have made currently available methods for automating plasmid DNA minipreps less than satisfactory. PhyNexus has developed a solution for obtaining reproducible automated plasmid DNA minipreps in a truly walk-away fashion. Lysate Direct PhyTip® Column technology consists of a purification column in a pipet tip format. These novel purification columns are composed of pipet tip containing plasmid DNA binding resin and a thin, inert frit screen at the end of the tip to retain the resin. The unique design of the column allows for DNA binding directly from cell lysates in the presence of precipitates and debris without sample clearing. By eliminating the need for centrifugation or filtration, Lysate Direct PhyTip® Columns for Plasmid DNA Purification enable utilization of liquid handing automation found in high throughput laboratories to easily purify up to 96 samples in parallel.

Documents
Compatibility

HTP Miniprep PhyTip Columns are available for these robotic systems.

Citations

Publications


Implementation of an Automated High-Throughput Plasmid DNA Production Pipeline
Billeci K, Suh C, Di Ioia T, Singh L, Abraham R, Baldwin A, Monteclaro S
Publication: Journal of Laboratory Automation, 2016
doi: 10.1177/2211068216630547
http://journals.sagepub.com/doi/pdf/10.1177/2211068216630547

Posters


Innovative Solutions for Automated Protein and Nucleic Acid Purification
Lee Hoang, PhyNexus, Inc., PhyNexus Webinar

Automated, High Throughput Protein Purification and Sample Prep Using PhyTip Columns for Therapeutic Leads Screening and Process Development
Lee Hoang, PhD., PhyNexus, Inc., The 5th Annual Proteins Congress 2012, London

PhyNexus Users Group Symposium 2014
Douglas Gjerde, PhyNexus
Lee Hoang, PhyNexus
Karen Billeci Genentech

Select your resin, robotic system, tip and resin volume and see the results in the table below.

HTP Miniprep PhyTip columns