PCR reactions generate DNA fragments of interest. However, the reaction product also consists of contaminants that include excess dNTPs, primers, salts and failure products. In a typical cleanup, the target double stranded DNA may be 2kb in length. PCR reactants and byproducts of 100 bp and smaller are eliminated. Yield and functionality are maintained while benefiting from high throughput automation. The purified PCR products are suitable for generating RNA by reverse transcription, cloning, hybridization reacts, and a host of other applications.
PhyNexus utilizes a proprietary chemistry to clean up PCR products in a fully automated, 96-at-a-time format. Depending on the sample, PhyNexus employs a number of different chemistries for performing automated PCR clean up. These include ion pairing and chaotropic precipitation. Gel filtration and ion exchange may also be considered. The chemistries are focused towards not only optimizing the method to minimize sample loss, but on the downstream application desired. Sample size and automated throughput are also considered.