Tail biopsy (tail snipping) for genomic analysis of mice is necessary for the identification and validation of genetically modified mice. The number of laboratory mice used as a model for human and animal biomedical research continues to grow at a rapid rate. Each mouse must be tested. Most often, several milligrams of solid tissue (mouse tail) are needed to identify the presence or absence of a segment of DNA in the animal. PhyNexus technology uses resin media that has an extremely high selectivity for genomic DNA allowing rapid and complete capture of genomic DNA from complex sample matrices. The capture columns tolerate the presence of particulate allowing faster sample preparation of the solid sample – as fast as 3 hours vs overnight digestion for traditional methods. The automated method may be integrated with other automated instruments, such as sequencers. The semi-automated plate method is the closest comparable technology.
Preclinical drug testing and other types of research are performed with transgenic mice. These mice have knockout genes or inserted genes to test the effect of drugs prior to human testing. The mouse’s usefulness as a biomedical model has been sharply enhanced by recent advances in the ability to experimentally modify its genome. About half the 50 million animals used in research today are genetically modified mice. This means there are 25 million/year and growing rapidly. Rapid growth is due increased ease to manipulate and humanize the mouse genome. Research is shifting to the use of genetically modified mice to study biological pathways and the effect of potential drugs. PhyTip columns perform better due to increased selectivity of the media to genomic DNA and the ability to operate in the presence of particulate.
Other Genomic DNA
The technology developed for mouse tail can be used in other genomic DNA samples types while implementing automation including bacterial cell pellets, live and dried cricket, live and dried worm, goldfish and any comparable sample. See your PhyNexus representative for details.