The PhyNexus MEA 2 is a fully automated protein purification system. It fills the productivity gap between the ME semi-automated instruments and 96-channel capability robots. When used with the unique PhyTip Cover, elution plates retain the integrity of the final elution at volumes as low as 10 μL, to guarantee high concentrations of ultra-high purity samples. The MEA 2 system offers the following:

  • Automated programming of a 12 channel system: Allows complete flexibility of protocols and applications with maximum throughput. Complete purification of 1-12 samples in parallel, 96 samples in as little as two hours.
  • Diverse applications: Purify proteins, antibodies and peptides. Run chromatographic resins such as reverse phase, normal phase and ion exchange. Process complicated biochemistry such as immunoprecipitation, co-immunoprecipitation, conjugations and on-column reactions. Prepare samples for analytics, leads screening, enzyme engineering, and target identification. Simultaneously prep twelve large samples of 60 mL starting volumes.
  • Cold room compatibility: Operate the MEA 2 in a cold box or cold room to ensure sample stability.
  • Easy-to-Use Software Programming:The PhyNexus software comes prepackaged with validated methods for antibody purification, purification of tagged proteins, ion exchange, reverse phase and normal phase chromatography. Users may opt for complete control of the MEA 2 for method and process development. The PhyNexus MEA 2 Operating Software allows the user to reproducibly move the 12-channel system and PhyTip columns to any position on the sample deck and provides complete control of flow rates and volumes. This software and programming flexibility allows the user to easily modify pre-programmed methods or develop complex novel methods that can be stored for future use. The software is designed to operate with PCs running Windows 8 and 10.
  • Complete automation: Completely automated, walk-away purification, chromatography, buffer exchange and desalting. Methods ensure reproducibility and scalability of results.
  • Easy conversion from 200 μL to 1000 μL system: Easily converts within minutes for use with either 200+ or 1000+ PhyTip columns.
  • Variable plate positions: Can be used with a range of different plate types and positions that give complete flexibility. There are two positions available for PhyTip column boxes or pipette tip boxes, four positions for sample, wash, and elution plates, and one position for a 250 µL buffer reservoir. Two plate positions are capable of cooling to 4°.
  • Flexibility: Configure the MEA 2 to process proteins. Methods are transferable to virtually any liquid handing instrument for increased throughput.
PhyNexus MEA 2 Fully Automated Protein Purification System

The MEA 2 system offers the following:

  • Automated 12-channel system: Full incorporation of the latest features in liquid management. The re-designed 12-channel head result from advanced engineering combined with customer-driven feed-back. Complete purification of 96 samples in as little as two hours. Process sample volumes of 100 μL to 60 mL. Interchangeable between 1000+ and 200+ μL heads.
  •  Easy-to-Use software programming with an intuitive user interface.
  • Streamlined and Configurable system: Streamlined computer and power connections, configurable for protein or any need.
  • Cold Room Operation: Upgraded 12-channel head compatible with cold room operation and surfactant-rich samples.
  • Turnkey Applications: Validated procedures for antibody purification, tagged and native protein purification and buffer exchange.
  • Method development and transfer: Develop methods and transfer to robots from Tecan, Caliper/Perkin Elmer, Beckman, Dynamic Devices, Agilent and Hamilton.

PhyTip Columns for PhyNexus MEA 2