Adeno-Associated virus (AAV) is a nonpathogenic human virus that is currently the leading candidate for gene therapy. The genome of this virus consists of a single-stranded linear DNA molecule of approximately 4.7 kb. The Adeno-associated virus is used as a vector for the delivery of various types of cargo to the cytoplasm and nucleus of various cells including muscle, vascular endothelial and neuronal cells.

AAV Plasmid Purification

Biotage PhyPrep automates the purification of plasmid DNA from cell pellets. The result is highly pure, endotoxin-free, supercoiled plasmid DNA for use in gene therapy applications.

Downstream AAV Manufacturing Challenges

For the downstream workflow of virus production, affinity chromatography purification has been found to effectively remove impurities such as plasmid DNA and host cell RNA/DNA. That is then followed by an anion exchange polish to remove empty capsids. However, column chromatography is a costly and time consuming process that may still not produce the desired results consistently each time. You can quickly save valuable time in your affinity purification by quickly performing process validation of your long chromatography step with the Biotage AAVX PhyTip columns in only 30 minutes.
Biotage-AAVX-column-workflow

CaptureSelect® AAVX

The CaptureSelect AAVX affinity matrix is specifically designed to address the high selectivity and capacity requirements for large-scale downstream purification of a broad range of naturally occurring and synthetic adeno-associated virus (AAV) serotypes used for gene therapy applications. POROS CaptureSelect AAVX resin has demonstrated binding reactivity towards a set of AAV serotypes that includes AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, and AAVrh10 as well as synthetic serotypes.

The Biotage AAV PhyTip columns with our proprietary Dual Flow Chromatography are compatible with most 8, 12, and 96-channel automation liquid handling robots including MEA2, Agilent TechnologiesBeckman CoulterDynamic DevicesHamilton, Opentrons, Perkin Elmer, and Tecan.

CaptureSelect® is a trademark of Thermo Fisher Scientific.

Biotage CaptureSelect AAVX column

CaptureSelect tips are available in tip volumes (µL) of 200|300|500|1000 and resin volumes (µL) of 5|10|20|40|80|160|320 depending upon robot configuration.

Select your resin, robotic system, tip and resin volume and see the results in the table below.