PhyNexus technology provides mini scale plasmid DNA purification in 96 well format. The recovered plasmid requires no desalting or precipitation, yet it is transient transfection quality. This is accomplished using the PhyTip Dual Flow Chromatography technology with a proprietary silica resin. Unlike anion exchange, which requires desalting or precipitation, the plasmid recovered from the PhyNexus process may be used for any application including transient transfection with zero endotoxin.
Samples are grown in 96 well format and processed into cell pellets. Suspension, lysis, precipitation, plasmid capture, and recovery is performed without centrifugation or removal of cell debris. The robust process is capable of performing regardless of sample variation (essential for automation). The technology is compatible with the major robotic liquid handling systems.