Plasmid DNA Purification | Miniprep, Maxiprep, Megaprep, Gigaprep

A New Perspective – A New Solution

PhyNexus looked at the challenge of automating plasmid purification from a new perspective and turned an industry practice on its head. The industry practice starts by placing limits on the size and density of plasmid culture used, unless it overwhelms the narrow parameters of the method. Then it offers a yield from an already limiting starting point. But growing large, rich plasmid DNA samples is anything but limiting.  So PhyNexus encourages customers to grow and process large rich plasmid DNA samples that are ideal for automated processing. As a result, PhyNexus is changing the way researchers and scientists think about the plasmids they are producing and purifying: it’s no longer a question of plasmid yield from limited manual processes, it’s a question of plasmid mass from easy automated processes.

To introduce automation to plasmid purification, PhyNexus uses its patented proprietary PhyTip Columns and Dual Flow Chromatography to purify the plasmid DNA. The PhyTip Columns draw the sample up into the resin bed where the plasmids are captured, washed, and eluted. From the outset, the process has been designed to be very robust to accommodate a variety of culture media, yet precise enough to deliver high quality endotoxin free plasmids.