PCR product samples were purified with PhyTip silica columns using a chaotropic separation mechanism. PCR products from 4 different amplicons were studied. For each amplicon, 200 µL of samples were purified and 100 µL of purified product recovered. The results are shown on the gel to the right.
Samples A, B, C, D, and E are differently sized amplicons. Sizes are shown with the ladder to the left. The 10 bp loading dye indicator is the reference marking the end of the gel. The control samples on the right show unpurified PCR product. The recovered purified samples are shown by the left columns. The result show that the PCR products recovered remain concentrated and are not diluted by the process.
The sample UV absorbances were measured with a NanoDrop spectrophotometer. The unpurified controls (starting samples ) recorded an A260/A280 ratio of 1.79-1.92 and an A260/A230 ratio of 1.26-1.47 indicating the presence of contaminants including salts and enzymes. The purified PCR products record an A260/A280 ratio of 1.8-2.0 and an A260/A230 ratio of 2.0-2.2 indicating pure DNA.