Bind wide range of proteins/peptides sizes (1 kDa to 26.6 kDa)
PhyNexus Delivers Superior Results with Ease of Automation
PhyNexus C18 PhyTip Columns enable a HTP automated protein and peptide clean-up process delivering a streamlined solution for mass spectrometry sample prep. C18 PhyTip Columns deliver excellent results with a variety of solvents, and are an ideal way to set up a small to mid-scale MALDI and/or ESI liquid chromatography MS workflow. And C18 PhyTip Columns work with virtually all major liquid handling instruments already on the market.
Mass Spec Sample Prep and the Need for an Automated Workflow
Mass spectrometry is a powerful tool for qualitative and quantitative analysis of peptides, proteins and protein complexes, and an increasingly important part of drug discovery and development. MS can accurately determine the mass and characterization of proteins, provide antibody mapping and peptide mapping for biomarker discovery, identify post-translational modifications, map protein complexes and give insight to protein-protein and protein-to-lipid interactions. As such, labs are analyzing ever more analytes across multiple screening conditions.
But sample prep for MS can be slow and labor intensive, involving analyte capture, purification and enrichment, and subsequent analyte clean-up of salts and surfactants – which are incompatible with MS – with C18 resins.
PhyNexus Delivers a HTP Sample Prep Solution for MS
PhyNexus C18 PhyTip Columns offer an automated solution, compatible with existing liquid handling robotics, to sample clean-up for MS. Moreover, as part of LC-MS workflow, C18 PhyTip Columns can be integrated with upstream sample purification and concentration steps on an HTP automated system to vastly reduce labor, increase throughput and allow for significant conditions screening variability.
PhyNexus C18 columns bind proteins and peptides through reverse phases solid phase extraction (SPE). The C18 PhyTip Columns contain one of two resin options between two inert screens at the column tip. The liquid handler (handheld, bench top, or HTP 96-channel) then processes the sample with the back and forth flow of Dual Flow Chromatography to drive the binding reaction to saturation. C18 PhyTip Columns deliver excellent results with all the benefits of automation.
Call (408) 267-7214 to discuss your application with one of our scientists.
PhyNexus Advantages
High absolute binding capacity
Low loss or peptide
Compatible with many MS solvent and acid combinations
Automatable on HTP or bench top
Compatible HTP Systems |
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Agilent Technologies |
Beckman |
Dynamic Devices |
Hamilton |
Perkin Elmer |
Tecan |
Products
Products Catalogs
Applications
- Plasmid DNA Purification | Miniprep, Midiprep, Maxiprep, Megaprep, Gigaprep
- Transient Transfection
- Diagnostics
- Protein Purification
- Method Development
- HTP Gel Filtration
- PCR Clean-up
- Mouse Tail Genomic DNA
- Mass Spectrometry Sample Clean-up
- Custom Resin Solutions
- Genentech | Karen Billeci | Implementation of High Throughput DNA Production Pipeline
PhyTip Columns for Robots
Technology
- Dual Flow Chromatography Technology
- General Technology Q&A
- Automated High Throughput Liquid Handling Robotic Systems Automation
- Automation and the Separation Process
- Protein Purification & Method Development
- Plasmid Transfection Quality Purification
- Plasmid and Genomic DNA Purification
- Plasmid Transfection Quality Purification
About PhyNexus
3670 Charter Park Dr # A
San Jose, CA 95136
TEL: (408) 267-7214
FAX: (408) 267-7346
Europe Sales & Support
TEL: +49 171 5573391