PhyNexus PhyTip Column technology is exceptionally well suited for diagnostic applications requiring miniaturized, automated processing. Virtually any type of diagnostic column chemistry can be adapted to the platform technology, and PhyTip Columns can deliver better results in terms of yield, concentration and purity.
Drivers for the shift to PhyTip Columns for diagnostic applications include:
- The need or desire to miniaturize. This lowers the amount of sample needed. This is becoming necessary for any new diagnostic method. In fact there is a drive to miniaturize existing method.
- The need or desire to automate, scale and adjust to sample load.
- The need or desire to perform on column reactions to tag analytes for detection. On column reaction are performed with active flow of reagents to the analyte to produce high concentrations and uniform concentrations of the tagged, detectable analyte. The reagent mass amounts needed for tagging are reduced because of the small column size and ability to adjust conditions to drive the reaction to completion. This lowers the cost of the method while improving the performance.
- The need or desire to reproducibly concentrate the sample from dilute sources. This is needed to increase signal and increase the signal to noise ratio
- The need or desire to efficiently and reproducibly remove background materials. This is need to lower the background signal and increase the signal to noise ratio.
Two types of analytical mechanisms are commonly employed. One mechanism involves capturing the analyte, washing residual matrix materials and contaminants away and then recovering the analyte for detection. At this point in the process, the analyte may be detected directly using spectroscopy or mass spectrometry. Or the analyte may be tagged with a reagent to make the analyte detectable. The tagging operation and detection may be incorporated into the robotics workflow.
On column reaction assays have been employed using PhyTip columns. In these cases, an enzyme is immobilized on the column. The sample is introduced into the column to react with the enzyme and produce a detectable analyte.