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Fully automated instrument for Maxiprep/Megaprep/Gigaprep plasmid purification. Miniprep for liquid handlers.
MEA 2 Personal Purification System is a fully automated system designed for protein purification.
Our PhyTip columns work with all 8, 12, and 96 channel liquid handling high throughput robotics.
PhyTip columns for protein and plasmid purification are available for all major HTP liquid handling high throughput robotics.
Poster: Fully Automated Sample Preparation Platform for Peptide Mapping and Protein Identification by Mass Spectrometry Publication: Semiautomated Small-Scale Purification Method for High-Throughput Expression Analysis of Recombinant Proteins. Book: High-Throughput Protein Production and Purification - Methods and Protocols Publication: Perfusion cell culture for the production of conjugated recombinant fusion proteins reduces clipping and quality heterogeneity compared to batch-mode processes. Publication: Salivary N-glycosyalation as a biomarker of Oral Cancer: A pilot study. Publication: Shedding Light into the Subcutis: A Mass Spectrometry Based Immunocapture Assay Enabling Full Characterization of Therapeutic Antibodies after Injection in Vivo.
“We have been using PhyTips to purify protein variants of several enzyme libraries in our high-throughput screening approach. The possibility of using automation protocols to obtain protein of high purity in 96-well format increased our throughput and efficiency immensely. PhyNexus’ customer service and technical support has been outstanding, and the staff has been responsive and very knowledgeable when helping us establish purification protocols. Moreover, PhyNexus was very supportive in addressing specific needs for our enzymes, providing highly reliable products of outstanding quality.”
“Ever since they started up, PhyNexus has been providing my research group with high quality separation media, in a format that continues to exceed our expectations. When combined with automation, their innovative approaches and impressively responsive support systems, have made us into loyal customers! For me PhyNexus epitomizes an organization that not only demonstrates innovation, but understands the challenges faced by the user/customer and provides solutions that are both powerful and great value for money.”
“Using the PNGase F functionalized microcolumns from PhyNexus, we obtained very efficient deglycosylation in 10 min with excellent reproducibility and the option for full automation. Using this approach is crucial if the presence of the digestion enzyme represents a problem after the glycan release, e.g., when the remaining protein is further interrogated by mass spectrometry. The support of PhyNexus during the development of the assay was exceptional.”
“PhyNexus tip technology is a very reliable and enabling technology for high throughput purification. The technical support and scientific expertise in this company is second to none”
“I use the PhyNexus MEA system for discovery research applications for over 10 years now. Immunoprecipitations for Western Blot, protein concentration or purification from tissue extracts and cell culture supernatants and more. The system is straight forward, versatile, reliable and completely adjustable to the conditions I need and I can change the protocols and methods easily to fit my needs. It works for high throughput equally well as for “never-done-before, give-it-a-try” assays. Plus, as far as I am concerned, they offer the best customer service on the planet!”
“Our team purifies glycoproteins from bloods and serum using PhyNexus tips, using both pre-packed tips and custom tips on an automated handling system. We observe repeatable, reproducible results and high purity every time. The advantage of using the tip technology over traditional plate formats for our application is that we can extract multiple proteins/glycoproteins from the one clinical sample, expanding our assays for life sciences discovery.”
PhyNexus produces columns for miniprep transient transfection grade plasmids up to 15μg at 96 samples at a time.
Use our desalting & buffer exchange gel filtration columns to process large numbers of antibody & protein leads.
Our DFC technology provides complete antibody binding for highest sample recovery yields with any sample or column size.
The PhyTip column technology is a platform technology that can be directed to many different applications.
