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Automated Protein and Plasmid Purification | PhyNexus
  • ProductsProducts
    • Automated Protein Purification
      • Antibody Binding PhyTip Columns
        • Protein A
        • Protein G
        • ProPlus/ProPlus LX (MabSelect™)
        • CaptureSelect® Affinity
      • Recombinant Protein PhyTip Columns
        • IMAC (His-tagged Proteins)
        • Glutathione (GST-tagged Proteins)
        • Streptavidin (Biotin-tagged Molecules)
      • Chromatography PhyTip Columns
        • Normal Phase
        • Ion Exchange
        • C4 Mass Spectrometry
        • C18 Mass Spectrometry
      • Gel Filtration Buffer Exchange
      • Custom-packed Resin
    • Nucleic Acids Products
      • Plasmids
        • HTP Plasmid DNA Miniprep
        • Maxi, Mega & Giga Prep
        • Miniprep (AutoPlasmid MEA)
      • Genomic DNA
        • HTP Mouse Tail, Cell and Tissue
      • HTP PCR Clean-up PhyTip Columns

      • Automation / Workflow Integration
        • Plasmid to Mammalian Protein
        • Culture Plasmid Purification
        • Glycan Analysis
    • Product Catalogs
      • PhyTip Columns
      • Accessories
      • Labware & Extra buffers
      • Instruments
      • Service Contracts


      • PhyTip Columns for Robotic Systems
      • Agilent
      • Analytik Jena
      • Beckman Coulter
      • Dynamic Devices
      • Hamilton
      • Opentrons
      • Perkin Elmer
      • Tecan
    • Instruments
      • AutoPlasmid MMG
      • PhyNexus MEA 2
      • PhyNexus ME


      • Custom Resin Packing Service
  • Robotics Automation
    • PhyNexus MEA 2
    • PhyNexus ME
    • Agilent
    • Analytik Jena
    • Beckman Coulter
    • Dynamic Devices
    • Hamilton
    • Opentrons
    • Perkin Elmer
    • Tecan
    • Contact Automation Specialist
  • TechnologyTechnology
    • Dual Flow Chromatography
    • Robotics Automation & PhyTip Columns
      • Liquid Handling Robots Automation
      • Protein Purification
      • Desalting & Buffer Exchange
      • Plasmid & Genomic DNA Purification
    • General Technology Q&A
      • Automation & the Separation Process
      • Protein Purification & Method Development
      • Plasmid Transfection Quality Purification
  • ApplicationsApplications
    • Plasmid DNA Purification
      • Maxi, Mega & Giga Prep AutoPlasmid MMG
      • Mini and Midi Scale Prep Benchtop 12-channel
      • Mini Prep Scale Prep HTP 96-at-a-time

      • Transient Transfection
      • Small Volume, High Concentration Automation

      • Diagnostics
      • DFC Platform for Diagnostics
    • Protein Purification
      • Affinity Pull Down
      • Immunoprecipitation / Co-immunoprecipitation
      • Chromatin Immunoprecipitation
      • Protein-Antibody Engineering
      • Target Discovery
      • DNA Encoded Library Screening
      • Pharmacokinetic / Pharmacodynamic Modeling
      • Immunogenicity
      • Quality Control
      • Library Screening (Fabs, scFvs, small molecules)
      • X-ray Crystallography, Membrane Proteins
      • Lead Optimization / Screening
      • Expression Optimization / Screening
      • Epitope Mapping

      • Method Development
      • Multi-parallel Formulation Screening
      • Protein Analytics & Characterization
      • Process Development / Continuous Chromatography
      • Glycan Profiling
    • Gel Filtration
      • HTP Desalting & Buffer Exchange

      • PCR Clean-up
      • PCR Clean-up using PhyTip Silica Columns

      • Mouse Tail Genomic DNA
      • Automated Mouse Tail Genomic DNA

      • Mass Spectrometry C18 & C4
      • C-18 and C-4 Resins
      • Gel Filtration Desalting

      • Custom Resin Solutions
      • Custom resin packing service
  • ResourcesResources
    • Application and Technical Notes
    • Posters and Presentations
    • Journal Publications & Books
    • Product Information Documents
    • Material Safety Data Sheets (MSDS)
    • Safety Data Sheets (SDS)
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PhyTip-columns-for-antibody-library-screening

Accelerate your

Coronavirus

research

for antibody library screening, therapeutic leads screening,

and neutralizing antibodies discovery.

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We specialize in automated

protein & plasmid purification.

Get up to

95% yields

with PhyTip Columns on your robot!

Any sample volume, any column size, scalable, and reproducible

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PhyNexus Customers AMGEN, MERCK, GSK, EMD SERONO, LILLY, GENENTECH

Our customers are

companies and research groups

that touch people’s lives.

PhyNexus AutoPlasmid MMGPhyNexus AutoPlasmid MMG

AutoPlasmid MMG

Fully Automated Plasmid Purification

Maxiprep, Megaprep and Gigaprep

Transfection Grade Plasmids

Hands-Free Walk Away

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WATCH THE VIDEO

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PhyNexus PhyTip ColumnsLearn More Button

We provide the chemistry to fit your automation research needs.

PhyNexus advanced manufacturing provides

exactly what you want when you need it.

We can pack virtually any resin into

all common robotic liquid handler pipette tips.

PhyNexus AutoPlasmid MMGGel Filtration columns

Fully Automated Gel Filtration for

Compatible with all major robotics

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Remove >95% of salts with >80% yield of protein

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Buffer Exchange & Desalting

96 sample format for HTP

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PhyNexus_automation_solutions

Want to

automate your application

and don't know where to start?

We can find you the right solution through our

partnerships with major robotics companies.

Learn More ButtonAutomation Robots

Automated Protein and Plasmid Purification with up to 95% Yields!

PhyNexus provides life science researchers automation solutions to high throughput sample processing in drug discovery and screening using our PhyTip columns with Dual Flow Chromatography technology.

PhyNexus-Automated-Plasmid-purification-Maxiprep-miniprep-megaprep-gigaprep

Automated Plasmid Prep

Fully automated instrument for Maxiprep/Megaprep/Gigaprep plasmid purification. Miniprep for liquid handlers.

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PhyTip-columns-Automated-Protein-Purification-Prep

Automated Protein Prep

MEA 2 Personal Purification System is a fully automated system designed for protein purification.

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PhyTip-HTP-automation-Columns-catalog

PhyTip Columns Catalog

Our PhyTip columns work with all 8, 12, and 96 channel liquid handling high throughput robotics.

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PhyTip columns for HTP automation 96 samples in 15 minutes

Automation Robotics

PhyTip columns for protein and plasmid purification are available for all major HTP liquid handling high throughput robotics.

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Poster: Fully Automated Sample Preparation Platform for Peptide Mapping and Protein Identification by Mass Spectrometry Publication: Semiautomated Small-Scale Purification Method for High-Throughput Expression Analysis of Recombinant Proteins. Book: High-Throughput Protein Production and Purification - Methods and Protocols Publication: Perfusion cell culture for the production of conjugated recombinant fusion proteins reduces clipping and quality heterogeneity compared to batch-mode processes. Publication: Salivary N-glycosyalation as a biomarker of Oral Cancer: A pilot study. Publication: Shedding Light into the Subcutis: A Mass Spectrometry Based Immunocapture Assay Enabling Full Characterization of Therapeutic Antibodies after Injection in Vivo.

PhyNexus-PhyTip-Dual-Flow-Chromatography-protein-plasmid-purification
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Compatible Robots

PhyNexus MEA 2

Agilent Technologies

Analytik Jena

Beckman Coulter

Dynamic Devices

Hamilton Robotics

Opentrons

Perkin Elmer

Tecan Robotics

Want to automate your application with robotics in your lab & don’t know where to start?

We have partnerships with major robotics companies & can find the right solution
for automating your high throughput applications.

We can provide the right automation solution for your application, provide method & protocol development, support, installation, and training.

Contact Automation Specialist

PhyNexus PhyTip Columns are compatible with most 8, 12, and 96-channel automation liquid handling robots including Agilent Technologies, Analytik Jena, Beckman Coulter, Dynamic Devices, Perkin Elmer, Hamilton, Tecan and Opentrons.

PhyNexus_Phytip_compatibility_with_Liquid_Handling_robots
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“We have been using PhyTips to purify protein variants of several enzyme libraries in our high-throughput screening approach. The possibility of using automation protocols to obtain protein of high purity in 96-well format increased our throughput and efficiency immensely. PhyNexus’ customer service and technical support has been outstanding, and the staff has been responsive and very knowledgeable when helping us establish purification protocols. Moreover, PhyNexus was very supportive in addressing specific needs for our enzymes, providing highly reliable products of outstanding quality.”

Dr. Barbara EckertPrincipal Scientist, Roche Molecular Systems, Inc., Diagnostics

“Ever since they started up, PhyNexus has been providing my research group with high quality separation media, in a format that continues to exceed our expectations. When combined with automation, their innovative approaches and impressively responsive support systems, have made us into loyal customers!  For me PhyNexus epitomizes an organization that not only demonstrates innovation, but understands the challenges faced by the user/customer and provides solutions that are both powerful and great value for money.”

Professor David HornbyDepartment of Molecular Biology and Biotechnology, University of Sheffield

“Our group has purified some of our proteins with your amazing tips. The result is fantastic! We are running binding assays and do not need much. The proteins have very good quality for binding assays.”

Dr. Heique BogdawaSenior Scientist at Phylogica, Drug Discovery Group, Perth, Australia

“Using the PNGase F functionalized microcolumns from PhyNexus, we obtained very efficient deglycosylation in 10 min with excellent reproducibility and the option for full automation. Using this approach is crucial if the presence of the digestion enzyme represents a problem after the glycan release, e.g., when the remaining protein is further interrogated by mass spectrometry. The support of PhyNexus during the development of the assay was exceptional.”

Professor András GuttmanHead of Horváth Csaba Laboratory of Bioseparation Sciences, University of Debrecen, Hungary

“PhyNexus tip technology is a very reliable and enabling technology for high throughput purification. The technical support and scientific expertise in this company is second to none”

Dr. Olivier DalmasScientist II, Biologics, NGM Biopharmaceuticals, USA

“I use the PhyNexus MEA system for discovery research applications for over 10 years now. Immunoprecipitations for Western Blot, protein concentration or purification from tissue extracts and cell culture supernatants and more. The system is straight forward, versatile, reliable and completely adjustable to the conditions I need and I can change the protocols and methods easily to fit my needs. It works for high throughput equally well as for “never-done-before, give-it-a-try” assays. Plus, as far as I am concerned, they offer the best customer service on the planet!”

Dr. Claudia FiegerResearch Scientist at Macrogenics, USA

“Our team purifies glycoproteins from bloods and serum using PhyNexus tips, using both pre-packed tips and custom tips on an automated handling system. We observe repeatable, reproducible results and high purity every time. The advantage of using the tip technology over traditional plate formats for our application is that we can extract multiple proteins/glycoproteins from the one clinical sample, expanding our assays for life sciences discovery.”

Dr. Roisin O’FlahertyPost Doctoral Research Fellow at National Institute for Bioprocessing Research and Training, Dublin, Ireland
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96 Plasmid DNA Miniprep Phytip Columns

Automated Miniprep

PhyNexus produces columns for miniprep transient transfection grade plasmids up to 15μg at 96 samples at a time.

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Hight Throughput Gel Filtration Desalting Columns

Automated Desalting Gel Filtration

Use our desalting & buffer exchange gel filtration columns to process large numbers of antibody & protein leads.

Learn More
PhyNexus Dual Flow Chromatography

Dual Flow Chromatography

Our DFC technology provides complete antibody binding for highest sample recovery yields with any sample or column size.

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PhyNexus PhyTips Columns applications

Applications

The PhyTip column technology is a platform technology that can be directed to many different applications.

Learn More

Products

  • PhyTip Columns Catalog
  • Automated Protein Purification
  • Nucleic Acids Products
  • Instruments
  • Custom Resin Packing Service
  • Gel Filtration & Buffer Exchange Columns
  • ProPlus/ProPlus LX Columns (MabSelect SuRe™)
  • IMAC Nickel Columns (Ni-IMAC)
  • C18 Mass Spectrometry Columns
  • C4 Mass Spectrometry Columns

Products Catalogs

  • Company and Products Overview
  • PhyTip Columns Catalog
  • Instruments Catalog
  • Accessories Catalog
  • Labware & Extra buffers
  • Service Contracts Catalog

Applications

  • Plasmid DNA Purification | Miniprep, Midiprep, Maxiprep, Megaprep, Gigaprep
  • Transient Transfection
  • Diagnostics
  • Protein Purification
  • Method Development
  • HTP Gel Filtration
  • PCR Clean-up
  • Mouse Tail Genomic DNA
  • Mass Spectrometry Sample Clean-up
  • Custom Resin Solutions
  • Genentech | Karen Billeci | Implementation of High Throughput DNA Production Pipeline

PhyTip Columns for Robots

  • Agilent Columns Catalog
  • Analytik Jena Columns Catalog
  • Beckman Coulter Columns Catalog
  • Dynamic Devices Columns Catalog
  • Hamilton Columns Catalog
  • Perkin Elmer Columns Catalog
  • Tecan Columns Catalog

Technology

  • Dual Flow Chromatography Technology
  • General Technology Q&A
  • Automated High Throughput Liquid Handling Robotic Systems Automation
  • Automation and the Separation Process
  • Protein Purification & Method Development
  • Plasmid Transfection Quality Purification
  • Plasmid and Genomic DNA Purification
  • Plasmid Transfection Quality Purification

About PhyNexus

  • Contact
  • Resources
  • News & Events
  • Journal Publications & Books
  • Posters and Presentations
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PhyNexus, Inc.
3670 Charter Park Dr # A
San Jose, CA 95136
TEL: (408) 267-7214
FAX: (408) 267-7346
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TEL: +49 171 5573391
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The following are trademarks of PhyNexus, Inc.: PHYNEXUS, PHYTIP, PHYCAP, CAPTURE PURIFY ENRICH, and TIP CONCENTRATING EFFECT.
Third party logos, trademarks, and registered trademarks are the properties of their respective owners.
©2021, PhyNexus, Inc., All Rights Reserved.
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