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Affinity Columns

Summary

  • Bidirectional flow allows maximum capture, purification and enrichment.
  • Capture, purify and enrich in as little as 15 minutes to obtain high concentrations of fully functional protein
  • Process small sample volumes in a reproducible, high throughput, automatable format
  • Elution volumes as low as 10 µL, producing enrichment factors as high as 50 fold, with concentrations of purified protein of up to 10 mg/mL

Products

Antibody binding:

Protein A

Protein G

ProPlus

His binding:

Ni-IMAC

GST binding:

Glutathione

Biotin binding:

Streptavidin

We also produce PhyTip columns containing Custom Resins

Standard Column Sizes

200 µL column with 5 or 20 µL resin

1000 µL column with 10, 20, 40, 80, 160 or 320 µL resin

Part Numbers

See Catalog for each Part Number. In summary:

PTR 92-(05 or 20)-0x, Box of 96, 200 µL, 5 or 20 µL resin bed

PTR 91-(10,20,40,80,160,320)-0x, Box of 96, 1000 µL, 10, 20 etc. µL resin bed

PTR 42-(05 or 20)-0x, Box of 48, 200 µL, 5 or 20 µL resin bed

PTR 41-(10,20,40,80,160,320)-0x, Box of 48, 1000 µL, 10, 20 etc. µL resin bed

x: 1- ProA, 2-ProG, 3-Ni-IMAC, 4-Glutathione, 5-Streptavidin, 7-ProPlus

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